LDH-A乙酰化在结直肠腺癌组织中的表达及意义  

Expression and significance of acetylated lactate dehydrogenase-A in colorectal cancer

作　　者：郑鸿 祝子华 ZHENG Hong;ZHU Zihua(Department of Chemotherapy,Affiliated Cancer Hospital Minhang Branch of Fudan Unversity,Shanghai 200240,China;Department of Gastroenterology,Affiliated Minhang Hospital of Fudan University,Shanghai 201199,China)

机构地区：[1]复旦大学附属肿瘤医院闵行分院化疗科,上海200240 [2]复旦大学附属闵行医院消化科,上海2011990

出　　处：《癌症进展》2018年第8期1008-1010,共3页Oncology Progress

基　　金：闵行区自然科学研究课题(2014MHZ069)

摘　　要：目的探讨乳酸脱氢酶A(LDH-A)的乙酰化在结直肠腺癌组织中的表达及其与结直肠腺癌患者临床特征的关系。方法应用免疫组织化学染色法检测40例结直肠腺癌患者的结直肠腺癌组织及其相应的40例癌旁正常组织中乙酰化LDH-A的表达情况。结果结直肠腺癌组织中乙酰化LDH-A蛋白的阳性表达率为22.5%(9/40),低于癌旁正常组织的47.5%(19/40),差异有统计学意义(P﹤0.05)。Ⅰ~Ⅱ期结直肠腺癌组织中乙酰化LDH-A蛋白的阳性表达率高于Ⅲ~Ⅳ期的结直肠腺癌组织,差异有统计学意义(P﹤0.05)。不同年龄、性别、分化程度结直肠腺癌患者的结直肠腺癌组织中乙酰化LDH-A蛋白的阳性表达率比较,差异均无统计学意义(P﹥0.05)。结论 LDH-A乙酰化水平在结直肠癌组织中呈低表达,提示其与结直肠癌的发生可能具有一定的关系,在结直肠癌发展中,LDH-A乙酰化可能起到负性调节的重要作用。Objective To investigate the expression of acetylated lactate dehydrogenase-A （LDH-A） in colorectal can- cer （CRC） and analyze its relationship with clinical features of CRC patients. Method Immunohistochemistry method was used to detect the expression of acetylated LDH-A in 40 specimens of CRC tissue and corresponding 40 specimens of adjacent non-cancerous tissues from 40 patients with CRC. Result The positive expression rates of acetylated LDH-A were 22.5% （9/40） in CRC tissues and 47.5% （19/40） in adjacent non-cancerous tissues, with statistically significant dif- ference （P〈0.05）. The expression rate of acetylated LDH-A in CRC tissue from patients with I-II stage CRC was signifi- cantly higher than that in CRC tissue from patients with Ill-IV stage CRC （P〈0.05）. But there was no significant differ- ences of positive expression rates of acetylated LDH-A in different age, sex, differentiated degree （P〉0.05）. Conclusion The expressions of acetylated LDH-A have decreased in CRC, which indicate that acetylated LDH-A maybe related to the development of CRC and play an important negative adjustment role in the initiation and progression of colorectal cancer.

关 键 词：乙酰化 乳酸脱氢酶A 结直肠腺癌 免疫组织化学染色 

分 类 号：R735.3[医药卫生—肿瘤]