肝细胞生长因子诱导非小细胞肺癌耐药研究  

Study on the Resistance of Non-small Cell Lung Cancer Induced by Hepatocyte Growth Factor

作　　者：刘丹丹[1] 王莉彦[1] 许尔屹[1] 赵伟华[1] LIU Dandan;WANG Liyan;XU Eryi;ZHAO Weihua(Department of Oncology,Heilongjiang Provincial Hospital,Harbin Heilongjiang 150036,China)

机构地区：[1]黑龙江省医院肿瘤科,黑龙江哈尔滨150036

出　　处：《中国卫生标准管理》2018年第17期97-99,共3页China Health Standard Management

摘　　要：目的探讨肝细胞生长因子诱导敏感非小细胞肺癌细胞对吉非替尼的耐药性及其机制。方法应用肝细胞生长因子分别诱导两种不同基因类型非小细胞肺癌细胞,依次开展细胞增殖、凋亡及细胞周期等检验,分析其对吉非替尼的耐药性及机制。结果 PC-9与H292细胞对吉非替尼的生长抑制效果与药物浓度呈负相关性,经肝细胞生长因子诱导后其半数抑制浓度明显上升。肝细胞生长因子在两种细胞对吉非替尼耐药方面有诱导作用,对PC-9细胞凋亡有明显影响,但对两种细胞周期均无显著影响,并且对两种肺癌细胞c-MET磷酸化均有刺激作用。结论肝细胞生长因子可诱导非小细胞肺癌细胞对吉非替尼耐药。Objective To investigate the resistance of hepatocyte growth factor（HGF） induced sensitive non-small cell lung cancer（NSCLC） cells to gefitinib and its mechanism. Methods Hepatocyte growth factor（HGF） was used to induce two different gene types of nonsmall cell lung cancer（NSCLC） cells. Cell proliferation, apoptosis and cell cycle tests were carried out in turn to analyze the resistance to gefitinib and its mechanism. Results The inhibitory effect of PC-9 and H292 cells on the growth of gefitinib was negatively correlated with the drug concentration, and half of the inhibitory concentration increased significantly after induction by hepatocyte growth factor. Hepatocyte growth factor（HGF） could induce gefitinib resistance in both kinds of cells, and had a significant effect on apoptosis of PC-9 cells, but had no significant effect on both cell cycles, and can stimulate c-MET phosphorylation in both kinds of lung cancer cells. Conclusion Hepatocyte growth factor can induce gefitinib resistance in non-small cell lung cancer cells by stimulating c-MET phosphorylation.

关 键 词：肝细胞生长因子 敏感非小细胞肺癌细胞 吉非替尼 肿瘤耐药 耐药机制 逆转耐药 

分 类 号：R734[医药卫生—肿瘤]