螯合anti—IL-1βmAb—SPIONs对颞叶癫痫模型的靶向诊治价值  

Targeting diagnosis and treatment value of chelating anti-IL-115 mAb-SPIONs in temporal lobeepilepsy model

作　　者：王艳丽[1] 胡喜斌[1] 高凌云[1] 吴兴饶 褚旭[1] 周树虎[1] 孔庆霞[1] Wang Yanli;Hu Xibin;Gao Lingyun;Wu Xingrao;Chu Xu;Zhou Shuhu;Kong Qingxica(Department of Neurology,Affiliated Hospital of Jining Medical University,Jining 272000,China)

机构地区：[1]济宁医学院附属医院神经内科,山东省济宁市272000

出　　处：《中华医学杂志》2018年第33期2678-2682,共5页National Medical Journal of China

摘　　要：目的探讨螯合anti。IL．1BmAb．SPIONs在氯化锂．皮罗卡品诱导的颞叶癫痫模型中的诊治价值。方法将45只颞叶癫痫模型大鼠按照随机数字表法随机平均分为生理盐水组、plain—SPIONs组、anti—IL-1βmAb—SPIONs组，各组于癫痫发作后3d和14d分别进行注射等量粒子，粒子注射前及注射后4h分别行MRI扫描并测量T，值。注射粒子14d后的SD大鼠，观察铁粒子在癫痫病灶的分布情况，观察各组神经元缺失、星形胶质细胞增生和小胶质细胞活化情况，并检测IL-1β、NF—KBp65在各组中的表达情况。结果癫痫大发作14d后，注射anti-IL-1βmAb．SPIONs后T：值84±14，明显低于生理盐水组，神经元缺失、星形胶质细胞增生、小胶质细胞活化现象明显改善，IL-1β、NF—KBp65表达也明显降低。结论anti—IL-18mAb—SPIONs可以透过血脑屏障对颞叶癫痫病灶进行靶向定位和治疗。Objective In order to study the diagnosis and treatment value of chelating anti-IL-1 β3 mAb-SPIONs in temporal lobe epilepsy model induced by lithium chlorid and pilocarpine. Methods Fortyfive temporal lobe epilepsy model rats were randomly and equally divided into saline group, plain-SPIONs group, anti-IL-1 β3 mAb-SPIONs group. Each group was injected with equal particles at day 3 and day 14 after the onset of seizures. MRI were conducteds before and 4 hours after particles injection and T： values were measured. The distribution of iron particles in the epileptic tissue was observed and the neuronal loss, astrocyte proliferation and microglia activation were detected. The expressions of IL-1 β3 and NF-KBp65 in each group were detected meanwhile. Results At day 14 after seizure, the value of T2 was 84 ＋ 14 after injecting anti-IL-1 β3 mAb-SPIONs. Compared with the control group, the value of T2 obviously declined. These phenomena of neuron loss, astrocyte proliferation and microglia activation had been improved obviously. IL-lβ3and NF-KBp65 expression also significantly reduced. Conclusion Anti-IL-lβ3 mAbSPIONs can penetrate blood brain barrier and plays an important role in targeting positioning and targeting therapy in temporal lobe epilepsy.

关 键 词：颞叶癫痫 IL-1β单克隆抗体 超顺磁性氧化铁纳米粒子 靶向定位 靶向治疗 

分 类 号：R742.1[医药卫生—神经病学与精神病学]