rmhTNF-α抑制胃癌细胞增殖作用机制的研究  

Study on the mechanism of rmhTNF-α inhibit the proliferation of human gastric cancer cell

作　　者：张春平[1] 代洪生 王津迪 潘光敏 高志星[2] 季万胜[2] ZHANG Chunping;DAI Hongsheng;WANG Jindi;Pan Guangmin;GAO Zhixing;JI Wansheng(Weifang Medical College,Weifang 261053,China;Department of Gastroenterology;Hospital Affiliated to Weifang Medical University,Weifang 261031,China)

机构地区：[1]潍坊医学院,山东潍坊261053 [2]潍坊医学院附属医院消化内科,山东潍坊261031

出　　处：《现代医学》2018年第7期748-750,共3页Modern Medical Journal

基　　金：山东省优秀中青年科学家科研奖励基金资助项目(BS2010SW034)

摘　　要：目的:探讨重组改构人肿瘤坏死因子-α(rmhTNF-α)抑制人胃癌细胞增殖的作用机制。方法:将不同浓度(0、50、100、200 U·ml^(-1))的rmhTNF-α作用于人胃癌细胞系MKN45(干预组),采用增殖/毒性检测试剂(CCK-8)测定细胞抑制率,实时荧光定量PCR测定Δ133p53、cjun、cfos mRNA表达变化。结果:细胞抑制率干预组高于对照组(rmhTNF-α0 U·ml^(-1)组),且随rmhTNF-α浓度的增加而增高(P<0.05);Δ133p53mRNA表达干预组较对照组低,且随rmhTNF-α浓度的增加而降低(P<0.05),cjun、cfos mRNA表达干预组较对照组高,且随rmhTNF-α浓度的增加而增高(均P<0.05)。Pearson相关分析显示,Δ133p53 mRNA与cjun和cfos mRNA的表达呈显著负相关(分别r=-0.954、-0.947,均P<0.01)。结论:rmhTNF-α可能是通过抑制Δ133p53表达并促进cjun、cfos表达而抑制胃癌细胞的增殖,其对Δ133p53的抑制作用可能是cjun、cfos介导的。Objective： To investigate the mechanism of recombinant mutant human tumor necrosis factor-α（rmhTNF-α） inhibit the proliferation of human gastric cancer cell. Methods： Human gastric cancer cell lines MKN45 were treated with different concentrations（0,50,100,200 U·ml^-1） of rmhTNF-α intervention group. The cell inhibition rate was detected by proliferation/toxicity detection kit（CCK-8）,and the expression of Δ133 p53,cjun,cfos mRNA were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction. Results： The cell inhibition rates in the intervention group was higher than that in the control group（rmhTNF-α 0 U·ml^-1 group） and it increased with the increasing of concentration of rmhTNF-α（P〈0. 05）; the expression of Δ133 p53 mRNA in the intervention group was lower than that in the control group and it decreased with the increasing of concentration of rmhTNF-α（P〈0. 05）; the expression of cjun,cfos mRNA in the intervention group were higher than those in the control group and they increased with the increasing of concentration of rmhTNF-α（all P〈0. 05）. Pearson correclation analysis showed the expression was negatively correlated markedly betweenΔ133 p53 mRNA and cjun,cfos mRNA（r =-0. 954,-0. 947,respectivly,all P〈0. 01）. Conclusion： rmhTNF may inhibit Δ133 p53 expression and promote cjun,cfos expression to further inhibit gastric cancer cell proliferation,the inhibitory effect on Δ133 p53 may mediated by cjun and cfos.

关 键 词：Δ133p53 cjun CFOS 重组改构人肿瘤坏死因子-α 胃癌细胞 增殖 

分 类 号：R735.2[医药卫生—肿瘤]