miR-193a和IL17RD在甲状腺癌中的表达及其临床意义  

Expression of miR-193a and IL17RD in thyroid cancer and its clinical significance

作　　者：刘美宏[1] 毕洁领[1] 唐文[1] LIU Meihong;BI Jieling;TANG Wen(Department of Mammary Gland and Thyroidism,Yibin Second People＇s Hospital,Yibin 644000,China)

机构地区：[1]宜宾市第二人民医院乳腺甲状腺科,四川宜宾644000

出　　处：《现代医学》2018年第7期765-769,共5页Modern Medical Journal

摘　　要：目的:探讨微小RNA-193a(miR-193a)和白细胞介素17受体D(IL17RD)在甲状腺癌组织中的表达及其与甲状腺癌临床病理特征的关系。方法:收集经病理学确诊为甲状腺癌(88例)、甲状腺腺瘤(31例)、桥本甲状腺炎(26例)、结节性甲状腺肿(12例)及正常甲状腺(18例)的冷冻组织,采用蛋白免疫印迹、实时定量PCR分析组织中miR-193a和IL17RD mRNA及其蛋白表达情况,并分析它们与甲状腺癌临床病理特征的关系。结果:与正常甲状腺组织相比,桥本甲状腺炎、甲状腺腺瘤、甲状腺癌组织中miR-193a表达水平显著降低,而IL17RD mRNA和蛋白表达显著增加(均P<0.05)。甲状腺癌组织中miR-193a和IL17RD蛋白表达水平与肿瘤大小、临床分期、组织学分化程度及淋巴结转移等临床特征有关(均P<0.05)。miR-193a与IL17RD mRNA和蛋白表达均呈明显负相关(P<0.05)。结论:甲状腺癌组织中miR-193a和IL17RD的表达与肿瘤大小、临床分期、组织学分化、淋巴结转移等特征有关,miR-193a可能通过靶向IL17RD参与甲状腺癌的发生及进展。Objective： To investigate the expression of miR-193 a and IL17 RD in thyroid carcinoma,and analyze the relationship with clinicopathological features of thyroid carcinoma. Methods： The frozen tissues from pathologically confirmed thyroid cancer（88 cases）,thyroid adenoma（31 cases）,Hashimoto＇ s thyroiditis（26 cases）,nodular goiter（12 cases） and normal thyroid gland（18 cases） were collected. Western blot,real-time quantitative PCR were used to analyze the expression of miR-193 a and IL17 RD in the tissues. The relationship with clinicopathological features of thyroid carcinoma was explored. Results： Compared with normal thyroid tissue,the expression of miR-193 a in Hashimoto＇ s thyroiditis,thyroid adenoma and thyroid cancer tissue were significantly decreased,while the expression of IL17 RD was significantly increased（all P〈0. 05）. The expression of miR-193 a and IL17 RD protein in thyroid cancer tissue were related to the clinical features such as tumor size,clinical stage,histological grade and lymph node metastasis（P〈0. 05）. There was a significant negative correlation between the expression of miR-193 a and IL17 RD（P〈0. 05）. Conclusion： The expression of miR-193 a and IL17 RD in thyroid cancer tissues are related to tumor size,clinical stage,histological differentiation,and lymph node metastasis. miR-193 a may be involved in the progression of thyroid cancer by targeting IL17 RD.

关 键 词：甲状腺癌 微小RNA-193a 白细胞介素17受体D 

分 类 号：R736.1[医药卫生—肿瘤]