Effect of electroacupuncture on expressions of acetylcholine and mucin 5AC in the lungs of rats with chronic obstructive pulmonary disease  

电针对慢性阻塞性肺疾病大鼠肺中乙酰胆碱及黏蛋白5AC表达的影响

作　　者：Cong Wen-juan Li Jing Liao Yu-jing Zhang Xin-fang Jiang Chuan-wei Xiang Shui-ying Huang Wen-biao Liu Xiao-yun Liu Zi-bing 丛文娟;李静;廖雨静;张新芳;江传玮;项水英;黄文彪;刘小云;刘自兵(Institute of Acu-moxibustion and Meridian,College of Acupuncture and Massage,Anhui University of Chinese Medicine,Hefei 230038,China;Department of Physiology,Integrative Medicine Institute,Anhui University of Chinese Medicine,Hefei 230038,China)

机构地区：[1]Institute of Acu-moxibustion and Meridian, College of Acupuncture and Massage, Anhui University of Chinese Medicine, Hefei 230038, China [2]Department of Physiology, Integrative Medicine Institute, Anhui University of Chinese Medicine, Hefei 230038, China

出　　处：《Journal of Acupuncture and Tuina Science》2018年第3期133-139,共7页针灸推拿医学（英文版）

基　　金：国家自然科学基金项目,No.81373743;No.81102660;安徽省自然科学基金.No.1408085MH201;安徽高校科研创新平台团队建设项目.No.2015TD033.

摘　　要：Objective： To observe the effect of electroacupuncture （EA） on the expressions of acetylcholine （ACh） and mucin 5AC （MUC5AC） in the lungs of rats with chronic obstructive pulmonary disease （COPD）, and explore the mechanism of EA in treating COPD. Methods： Thirty Sprague-Dawley （SD） rats were randomly divided into a control group, a COPD group, and an EA group, with 10 rats in each group. The control group was a group of normal rats. The COPD rat model was induced by cigarette smoke combined with lipopolysaccharide （LPS）. The COPD rats were treated with EA at bilateral Feishu （BL 13） and Zusanli （ST 36） in the EA group, 30 rain each time, once a day, successively for 14 d. The lung function was tested. The contents of ACh and MUC5AC in lungs and bronchoalveolar lavage fluid （BALF） were detected by enzyme-linked immunosorbent assay （ELISA）. Pearson method was used to analyze the correlation between pulmonary function and the content of MUC5AC in lungs. The mRNA and protein expressions of MUC5AC in lung tissues were detected by real-time polymerase chain reaction （RT-PCR） and Western blot （WB）, respectively. The immune response of MUC5AC was observed by immunohistochemistry. Results： Eight rats were left in each group, and the other two died. Compared with the control group, the total airway resistance （Raw） increased significantly and dynamic compliance （Cdyn） decreased significantly in the COPD group （P〈0.01）; compared with the COPD group, the Raw level declined significantly and Cdyn increased significantly in the EA group （P〈0.01）. The contents of ACh and MUC5AC in the lungs and BALF were remarkably higher in the COPD group compared with those in the control group （P〈0.01, P〈0.001）; compared with the COPD group, the contents of ACh and MUC5AC were significantly lower in the EA group （P〈0.05, P〈0.001）. There was a negative correlation between MUC5AC content and lung function （P〈0.001）. The mRNA and protein expressions 目的：观察电针对慢性阻塞性肺疾病（COPD）大鼠肺中乙酰胆碱（ACh）TZ黏蛋白SAC（MUCSAC）表达的影响，探讨针刺治疗COPD的作用机制。方法：将30只Sprague-Dawley（SD）大鼠随机分为对照组、COPD组和电针组，每组10只。采用香烟烟雾联合脂多糖诱导方法制备COPD模型。电针组大鼠予以电针双侧肺俞和足三里，每日1次，每次30min，连续治疗14d。干预结束后检测肺功能，应用ELISA法检测肺组织及支气管肺泡灌洗液（BALF）中ACh及MUC5AC的含量，采用Pearson法分析肺功能与MUCSAC含量的相关性，实时聚合酶链反应（RT-PCR）检测肺组织MUC5ACmRNA的表达，蛋白免疫印迹（WB）EL免疫组化方法检测MUC5AC的蛋白表达及免疫反应性。结果：每组均有2只大鼠死亡，剩余8只。与对照组比较，COPD组总气道阻力（Raw）显著升高且动态顺应性（Cdyn）显著降低（P〈0．01）；与COPD组比较，电针组Raw显著下降，Cdyn显著升高（P〈0．01）。与对照组比较，COPD组大鼠肺组织及BALF中ACh及MUC5AC含量均显著升高（P〈0．01，P〈0．001）；与COPD组比较，电针组ACh及MUC5AC含量均显著降低（P〈0．05，P〈0．001）。肺功能与MUC5AC含量呈负相关（P〈0．001）。与对照组比较，COPD组大鼠肺组织中MUC5AC的mRNA及蛋白表达均显著升高（P〈0．001）；与COPD组比较，电针组MUC5AC的mRNA及蛋白表达均显著下降（P〈0．01）。与对照组比较，气道上皮MUC5AC免疫反应活性显著升高（P〈0．001）；与COPD组比较，电针组气道上皮MUC5AC免疫反应活性显著下降（P〈0．001）。结论：电针可改善COPD大鼠肺功能，其机制可能与电针下调肺组织中ACh及MUC5AC的表达及降低气道黏液高分泌有关。

关 键 词：Acupuncture Therapy ELECTROACUPUNCTURE Pulmonary Disease Chronic Obstructive POINT Feishu （BL 13） POINT Zusanli （ST 36） ACETYLCHOLINE Mucin 5AC 

分 类 号：R2-03[医药卫生—中医学]