喉鳞状细胞癌组织中OCT-4、CD44的表达及OCT-4 CD44双阳性的临床病理学意义  

Expressions of OCT-4 and CD44 in laryngeal squamous cell carcinoma and the clinicopathological significance of OCT-4 and CD44 double positive

作　　者：包建涛[1] 颉玉胜[2] 王向红[1] 廖辉[3] 付莉[4] Bao Jiantao;Xie Yusheng;Wang Xianghong;Liao Hui;Fu Li(Department of Out-patient,Anning Hospital,General Hospital of Lanzhou Military Region,Lanzhou 730070,China;Department of Dermatology,A nning Hospital,General Hospital of Lanzhou Military Region,Lanzhoa 730070,China;Department of Otorhinolaryngology,Arming Hospital,General Hospital of Lanzhou Military Region,Lanzhou 730070,China;Department of Pathology,Arming Hospital,General Hospital of Lanzhou Military Region,Lanzhou 730070,China)

机构地区：[1]兰州军区兰州总医院安宁分院门诊部,730070 [2]兰州军区兰州总医院安宁分院皮肤科,730070 [3]兰州军区兰州总医院安宁分院耳鼻咽喉科,730070 [4]兰州军区兰州总医院安宁分院病理科,730070

出　　处：《肿瘤研究与临床》2018年第8期526-530,535,共6页Cancer Research and Clinic

摘　　要：目的检测核转录因子OCT-4在喉鳞状细胞癌组织中的表达及其与CD44表达的关系，进而探讨OCT-4与肿瘤干细胞的关系及其在肿瘤组织中表达的意义。方法筛选兰州军区兰州总医院病理科2008年1月至2012年12月鳞状细胞癌患者手术切除存档的喉癌标本69例。另取18例正常喉黏膜组织标本作为正常对照。利用组织芯片技术、免疫组织化学染色及免疫组织化学双重染色技术，定位标记OCT-4、CD44和OCT-4 CD44双阳性细胞，观察分析其在喉鳞状细胞癌组织中的表达数量、分布方式、细胞形态特征以及与患者临床病理参数的关系。结果喉鳞状细胞癌组织中CD44阳性细胞百分数为（63.62±8.76）%，高于正常喉黏膜的（13.61±5.81）%（P〈0.001）；喉鳞状细胞癌组织中OCT-4阳性细胞百分数为（50.44±10.84）%，而在正常喉黏膜组织中不表达（P〈0.001）；喉鳞状细胞癌组织中OCT-4阳性细胞百分数低于CD44（P〈0.001）。喉鳞状细胞癌组织OCT-4 CD44双阳性细胞百分数为（5.07±2.90）%。中、低分化喉鳞状细胞癌组CD44、OCT-4阳性细胞百分数分别为62.78%（8.22%）、49.89%（13.12%），高于高分化组[28.62%（30.45%）、33.00%（20.00%），P〈0.05]；在肿瘤干细胞中，中、低分化喉癌组的OCT-4 CD44双阳性细胞百分数高于高分化组[6.54%（4.24%）比1.18%（4.66%），P〈0.01]，且分化程度越低，OCT-4的表达水平越高；中、低分化组OCT-4和CD44的表达在不同T分期之间差异均无统计学意义（均P〉0.05），但OCT-4 CD44双阳性细胞百分数随着T分期的增加而不断升高（P〈0.05）；伴有淋巴结转移组OCT-4阳性细胞百分数高于无淋巴结转移组（P〈0.01）。结论OCT-4、CD44的表达与喉鳞状细胞癌的恶性生物学行为密切相关。OCT-4可能是喉鳞状细胞癌肿瘤干细胞有价值的标志物。ObjectiveTo detect the expression of nuclear transcription factor OCT-4 in laryngeal squamous cell carcinoma （LSCC） and its relationship with CD44, and to explore the relationship between OCT-4 and the cancer stem cells and its significance in tumors.MethodsSixty-nine specimens of laryngeal surgical resection were selected from LSCC patients from January 2008 to December 2012 in Lanzhou General Hospital of Lanzhou Military Region. Another 18 cases of normal laryngeal mucosa tissues were selected as control. OCT-4, CD44 and OCT-4＋ CD44＋ cells were observed by using tissue microarray, immunohistochemistry staining and immunohistochemical double staining techniques, and the relationship between their pathological features, such as expression intensity, distribution, cell morphology and clinical parameters were analyzed to explore the clinicopathological significance of CD44＋ OCT-4＋ cells.ResultsThe percentage of CD44-positive cells in LSCC was significantly higher than that in normal laryngeal mucosa tissues [（63.62±8.76）% vs. （13.61±5.81）%, P 〈 0.001]. The percentage of OCT-4-positive cells in LSCC was （50.44±10.84）%, while it was not expressed in normal laryngeal mucosa tissues （P 〈 0.001）. In LSCC, the expression of OCT-4 was significantly lower than that of CD44 （P 〈 0.001）; the coexpression rate of these two molecules was （5.07±2.90）%. The percentage of CD44-positive cells in poorly differentiated LSCC was significantly higher than that in the well-differentiated group [62.78% （8.22%） vs. 28.62% （30.45%）, P 〈 0.05]. The percentage of OCT-4-positive cells in poorly differentiated LSCC was significantly higher than that in the well-differentiated group [49.89% （13.12%） vs. 33.00% （20.00%）, P 〈 0.05]. In the cancer stem cells, the percentage of OCT-4＋ CD44＋ cells in poorly differentiated carcinoma group was also significantly higher than that in the well-differentiated carcinoma group （P 〈 0.01）, the lower the degree of differentiation, th

关 键 词：喉鳞状细胞癌 OCT-4 CD44 肿瘤干细胞 

分 类 号：R739.65[医药卫生—肿瘤]