蛋白酪氨酸磷酸酶SHP-1高表达对食管鳞状细胞癌细胞侵袭性和化疗敏感性的影响  

Effect of high expression of protein tyrosine phosphatase SHP-1 on the tumor cell invasiveness and chemosensitivity of esophageal squamous cell carcinoma

作　　者：费新雄 蒋忠君[1] 王钢胜 胡文兵[1] 陈曦[1] Fei Xinxiong;Jiang Zhongjun;Wang Gangsheng;Hu Wenbing;Chen Xi(Department of Internal Medicine of Thoracic Tumor,Edong Health Care,Huangshi Central Hospital,Affiliated Hospital of Hubei Polytechnic University,Puai Hospital,Wuhan 435000,China)

机构地区：[1]鄂东医疗集团黄石市中心医院(湖北理工学院附属医院)普爱院区胸部肿瘤内科,435000

出　　处：《肿瘤研究与临床》2018年第7期447-450,455,共5页Cancer Research and Clinic

基　　金：黄石市医药卫生科研项目（2014-03）

摘　　要：目的 探讨蛋白酪氨酸磷酸酶SHP-1高表达对食管鳞状细胞癌肿瘤细胞侵袭性和化疗敏感性的影响.方法 食管鳞状细胞癌EC9706细胞共分为3组,空白组未经任何处理;实验组采用SHP-1 mimic瞬时载体转染细胞24h,使用10 μmol/L顺铂再处理细胞12h;对照组采用10 μmol/L顺铂处理细胞12h.采用四甲基偶氮唑盐（MTT）法检测细胞增殖情况,Transwell小室法检测细胞侵袭性,实时荧光定量聚合酶链反应检测SHP-1 mRNA相对表达量,蛋白质印迹法检测SHP-1蛋白的表达水平.结果 实验组SHP-1 mRNA相对表达量（3.42±0.14）高于对照组与空白组（1.09±0.13、0.42±0.24,F=9.143,P＜ 0.05）.实验组与对照组、空白组相比,细胞生长抑制率提高、细胞凋亡指数增加（均P＜0.05）,组间两两相比差异均有统计学意义（均P＜0.05）.实验组、对照组与空白组的细胞侵袭率分别为（6.5±1.3）％、（18.5±2.5）％和（45.2±7.2）％,差异有统计学意义（F=11.853,P＜0.05）.结论 SHP-1高表达能抑制食管鳞状细胞癌肿瘤细胞的侵袭性,提高化疗敏感性,促进细胞凋亡,抑制细胞增殖,可为提高食管鳞状细胞癌化疗疗效奠定一定的理论基础.Objective To investigate the effect of high expression of protein tyrosine phosphatase SHP-1 on the tumor cell invasiveness and chemosensitivity of esophageal squamous cell carcinoma.Methods EC9706 ceils of esophageal squamous cell carcinoma were divided into three groups：the blank group was not given any treatment;the experimental group used SHP-1 mimic instantaneous vector to transfect cells for 24 h,and used 10 μmol/L cisplatin to treat EC9706 cells for 12 h;the control group selected 10 μmol/L cisplatin to treat EC9706 cells for 12 h.Cell proliferation was detected by using methyl thiazolyl tetrazolium （MTT） assay,and cell invasion was detected by using Transwell chamber.The expression of SHP-1 mRNA was detected by using fluorescence quantitative polymerase chain reaction.The expression level of SHP-1 protein was detected by using Western blot.Results The expression of SHP-1 mRNA in the experimental group （3.42t0.14） was higher than that in the control group and the blank group （1.09±0.13,0.42±0.24,F =9.143,P 〈 0.05）.Compared with the control group and the blank group,the cell growth inhibition rate and the apoptosis index in the experimental group were increased （both P 〈 0.05）,and the differences between any two groups were also statistically significant （all P 〈 0.05）.The cell invasion rate in the experimental group,the control group and the blank group was （6.5±1.3） %,（18.5±2.5） % and （45.2±7.2） %,respectively,and the difference was statistically significant （F =11.853,P 〈 0.05）.Conclusion High expression of SHP-1 can inhibit the invasion of esophageal squamous cell carcinoma,improve chemosensitivity,promote apoptosis and inhibit cell proliferation,which could lay a theoretical foundation for improving the efficacy of chemotherapy for esophageal squamous cell carcinoma.

关 键 词：食管肿瘤 蛋白酪氨酸磷酸酶 SHP-1 化学疗法 敏感性 

分 类 号：R735.1[医药卫生—肿瘤]