新型齿科可切削复合树脂陶瓷体外细胞相容性研究  

Cytocompatibility of new dental CAD/CAM composite resin-ceramics in vitro

作　　者：殷隽雅 曾剑玉[2] 张思佳 鲍艳娜[4] 司文捷[5] YIN Jun-ya;ZENG Jian-yu;ZHANG Si-jia;BAO Yan-na;SI Wen-jie(Department of Prosthodontics,Capital Medical University School of Stomatology,Beijing 100050,China)

机构地区：[1]宣化平安医院 [2]首都医科大学口腔医学院修复科,北京100050 [3]北京兴业口腔医院 [4]北京市昌平区中西医结合医院口腔科 [5]清华大学材料学院

出　　处：《北京口腔医学》2018年第4期181-185,共5页Beijing Journal of Stomatology

基　　金：首都医科大学基础-临床科研合作重点课题(17JL09)

摘　　要：目的初步评价新型齿科可切削复合树脂陶瓷的细胞相容性。方法依据国标和ISO相关标准制作CAD/CAM复合树脂陶瓷(UR)、CAD/CAM氧化锆(UZ)、光固化复合树脂陶瓷(SC)及各自与树脂粘接剂结合体标准试件(UR+、UZ+、SC+)的浸提液,并与L-929小鼠成纤维细胞共培养,1、3、5d后使用CCK-8法进行细胞增殖试验,评价各材料浸提液对细胞形态和增殖能力的影响。结果 UZ、UZ+、UR和SC组细胞在培养各时间段内都表现出较高的细胞增殖率,在第5d分别表现为95.1%、81.2%、87.1%和75.1%。UR+和SC+在培养5d后的细胞增殖率分别为70.6%和58.4%,两组光吸收度值分别为1.161和0.960,与阴性对照组1.645差异具有统计学意义,其中SC+组部分细胞镜下观察到边界模糊的形态。结论新型齿科可切削复合树脂陶瓷具有良好的细胞相容性。Objective To evaluate the cytocompatibility of new dental CAD/CAM composite resin-ceramics.Methods According to GB and ISO standards,standard test pieces of CAD/CAM composite resin-ceramics（ UR）,CAD/CAM zirconia（ UZ） and light-cured composite resin-ceramics（ SC） and their extract with resin adhesive were prepared and incubated with L-929 cell for 1 day,3 and 5 days. Then the morphology and proliferation of the cells were evaluated by the cell proliferation test（ CCK-8 test）. Results UZ,UZ ＋,UR and SC showed a high rate of cell proliferation during the whole period of time. The cell proliferation rates of UR ＋ and SC ＋ were 70. 6% and 58. 4% after five days. Significant difference was found in the OD values between these two groups and the negative control group. Some cells margin became fuzzy in SC ＋ group. Conclusion The new dental CAD/CAM composite resin-ceramics has good cytocompatibility.

关 键 词：可切削复合树脂陶瓷 细胞相容性 

分 类 号：R783.1[医药卫生—口腔医学]