Bioinspired Water-soluble Polymers with Grafted Polyamine Chains： Synthesis and Complexation with Oligonucleotides  

作　　者：Vadim V.Annenkov Uma Maheswari Krishnan Viktor A.Pal'shin Stanislav N.Zelinskiy Gayathri Kandasamy Elena N.Danilovtseva 

机构地区：[1]Limnological Institute of the Siberian Branch of the Russian Academy of Sciences, 3, Ulan-Batorskaya St., P.O. Box 278, Irkutsk, 664033, Russia [2]Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), School of Chemical and Biotechnology, SASTRA University, Thanjavur - 613401, Tamil Nadu, India

出　　处：《Chinese Journal of Polymer Science》2018年第10期1114-1122,共9页高分子科学（英文版）

基　　金：financial support from a joint grant of the Russian Science Foundation(#6-45-02001);the Department of Science Technology of the Ministry of Science and Technology of the Republic of India(#INT/RUS/RSF/10)

摘　　要：The siliceous frustules of diatom algae contain complex proteins known as silaffins, which consist of a peptide chain with grafted polyamine chains. These polyamines contain twenty or more nitrogen atoms with trimethylene groups between the nitrogens. We synthesized a set of polymers containing grafted long-chain polyamine fragments by using acryloyl chloride （ACh） polymers and activated acrylic acid copolymers as the starting materials. The new polymers contained 0.05 mol%-3.2 mol% of polyamine chains, which corresponded to 0.06-3.56 mmol·g- 1 amine groups. The new amine-containing polymers formed complexes with short （19-21-mer） deoxyribonucleic acid （DNA） and ribonucleic acid （RNA） strands, and these complexes penetrated into model yeast cells and A549 lung cancer cell. This study demonstrates the potential of these species based on long-chain polyamines to serve as novel gene delivery systems.The siliceous frustules of diatom algae contain complex proteins known as silaffins, which consist of a peptide chain with grafted polyamine chains. These polyamines contain twenty or more nitrogen atoms with trimethylene groups between the nitrogens. We synthesized a set of polymers containing grafted long-chain polyamine fragments by using acryloyl chloride （ACh） polymers and activated acrylic acid copolymers as the starting materials. The new polymers contained 0.05 mol%-3.2 mol% of polyamine chains, which corresponded to 0.06-3.56 mmol·g- 1 amine groups. The new amine-containing polymers formed complexes with short （19-21-mer） deoxyribonucleic acid （DNA） and ribonucleic acid （RNA） strands, and these complexes penetrated into model yeast cells and A549 lung cancer cell. This study demonstrates the potential of these species based on long-chain polyamines to serve as novel gene delivery systems.

关 键 词：POLYAMINE Polymeric amine Gene delivery Acryloyl chloride 

分 类 号：O631[理学—高分子化学]