分子印迹固相萃取-HPLC检测食品中痕量邻苯二甲酸酯类  

Molecularly imprinted solid phase extraction-HPLC for determination of trace phthalate esters in food

作　　者：韦寿莲 卢瑶 刘君红[2] 欧阳壮 魏承炀 WEI Shou-lian;LU Yao;LIU Jun-hong;OUYANG-Zhuang;WEI Cheng-yang(School of environmental and chemical engineering,Zhaoqing University,Zhaoqing 526061;Baotou Iron ＆ Steel Vocational Technical College,Baotou 014010;Institute of Zhaoqing Quality Supervision and Inspection,Zhaoqing 526070)

机构地区：[1]肇庆学院环境与化学工程学院,肇庆526061 [2]内蒙古包头钢铁职业技术学院,包头014010 [3]肇庆市质量计量监督检测所,肇庆526070

出　　处：《中国食品添加剂》2018年第8期201-206,共6页China Food Additives

基　　金：肇庆市科技计划项目(2015B010301002);"攀登计划"广东大学生科技创新培育专项资金(pdjh2017b0547)

摘　　要：报道一种固相萃取-高效液相色谱法(SPE-HPLC)检测塑料瓶装水、白酒和饮料中邻苯二甲酸酯类化合物(PAEs)。以邻苯二甲酸二辛酯(DOP)印迹聚合物为吸附剂,采用固相萃取法萃取复杂基体中PAEs,考察优化了萃取参数,包括样品pH和流速、淋洗剂的种类和体积、洗脱剂的种类和体积。在优化的萃取条件下,PAEs浓度的线性范围为0.10~100μg/mL,检出限(S/N=3)为0.03~0.05μg/mL,方法在塑料瓶装饮料3个水平DOP加标测定中显示好的精密度(RSD<5.5%)和高的回收率(88.7~97.2%)。A method was reported for the determination of phthalic acid ester compounds（PAEs）in plastic bottled water,wine and beverage samples by solid phase extraction-high performance liquid chromatography（SPEHPLC）. The PAEs were extracted from complex matrixes by solid phase extraction（SPE）method using dioctyl phthalate（DOP）imprinted polymer as the sorbet. Several parameters including pH of the sample solution and column flow rate,the type and volume of the elution solvent,and its composition and volume were investigated and optimized. Under the optimized extraction conditions,the linear range of PAEs concentrations was 0.10-100 μg/mL. The limit of detection（s/N=3）was 0.03 ~ 0.05 μg/mL. The method showed a good precision with relative standard deviations less than 5.5% and high recoveries between 88.7 and 97.2% for three different concentrations of DOP plastic bottled beverage samples.

关 键 词：印迹聚合物 固相萃取 邻苯二甲酸二辛酯 塑料瓶装物质 

分 类 号：TS202.3[轻工技术与工程—食品科学] TS207.3[轻工技术与工程—食品科学与工程]