增强化学发光酶免疫法测定血清高尔基蛋白73方法的建立及应用  

Establishment and application of an enhanced chemiluminenescence enzyme immunoassay to determine Golgi Protein 73 in serum

作　　者：肖静 陈文莉[2] 胡龙波 龙飞 彭涛 XIAO Jing;CHEN Wen-li;HU Long-bo;LONG Fei;PENG Tao(State Key Laboratory of Respiratory Disease,Guangzhou Hoffmann Institute,College of Basic Sciences,Guangzhou Medical University,Guangzhou,Guangdong 511436,China)

机构地区：[1]广州医科大学基础学院广州霍夫曼免疫研究所呼吸疾病国家重点实验室,广东广州511436 [2]广东省人民医院广东医学科学研究所感染科,广东广州510080

出　　处：《中国卫生检验杂志》2018年第16期1933-1937,共5页Chinese Journal of Health Laboratory Technology

基　　金：广州市科创委2016年科学研究专项一般项目(2016-0710388);广州市健康医疗协同创新重大专项(2016040-20012);广东省中国科学院全面战略合作项目(2012B0-90400012);中国科学院青年创新促进会项目(2013233)

摘　　要：目的建立对碘苯酚增强化学发光酶免疫(CLEIA)测定血清高尔基蛋白73(serum Golgi Protein,sGP73)的方法,用于检测人sGP73含量。方法建立增强CLEIA测定血清GP73含量,对一批临床样品包括重症肝炎患者30份,肝硬化患者38份,肝癌患者79份以及健康人43份,进行sGP73含量的测定。结果该方法检测人sGP73的灵敏度为0.197 ng/ml,线性范围为3.90 ng/ml^250.00 ng/ml。重症肝炎患者sGP73含量为8.98 ng/ml^1 393.00 ng/ml,肝硬化患者sGP73含量为14.36 ng/ml^599.70 ng/ml,肝癌患者sGP73含量为26.02 ng/ml^1 288.00 ng/ml,健康人sGP73含量为2.67 ng/ml^139.20 ng/ml。肝病患者远高于健康人sGP73含量,其中重症肝炎sGP73含量最高,肝癌患者高于肝硬化患者sGP73含量。结论成功建立增强CLEIA测定血清GP73含量的方法,与常规ELISA相比,具有更高的灵敏度,可作为肝癌早期诊断的重要辅助手段之一。Objective A para-iodophenol enhanced chemiluminenescence enzyme immunoassay（ CLEIA） was established for detecting the content of Golgi Protein 73（ sGP73） in serum. Methods The enhanced CLEIA system was developed to determine the sGP73 content in patients,including 30 cases of severe hepatitis,38 cases of liver cirrhosis,79 cases of hepatocellular carcinoma and 43 healthy controls. Results The assay sensitivity was 0. 197 ng/ml,with the linear range of standard curve3. 90 ng/ml-250. 00 ng/ml. The sGP73 levels in the serious hepatitis,liver cirrhosis,hepatocellular carcinoma and healthy controls were within 8. 98 ng/ml-1 393. 00 ng/ml,14. 36 ng/ml-599. 70 ng/ml,26. 02 ng/ml-1 288. 00 ng/ml and2. 67 ng/ml-139. 20 ng/ml,respectively. The sGP73 content in patients with liver disease is much higher than that of healthy people,with the highest in severe hepatitis,and the sGP73 content in liver cancer patients is higher than that in cirrhosis patients. Conclusion An enhanced CLEIA for quantitative measurement of sGP73 has been established,which showed higher sensitivity when compared with conventional ELISA. It is one of the adjuvant markers in the diagnosis of HCC.

关 键 词：化学发光酶免疫法 高尔基蛋白73 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]