一株2,2’,4,4’-四溴联苯醚(BDE-47)厌氧降解菌的筛选鉴定及降解特性  

Isolation, identification, and the degradation characteristics of a BDE-47 degrading strain

作　　者：谢苗苗 吴志能 王曦 顾捷 陈林 王莹莹[1] XIE Miaomiao;WU Zhineng;WANG Xi;GU Jie;CHEN Lin;WANG Yingying(Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria,Tianjin Key Laboratory of Environmental Remediation and Pollution Control,College of Environmental Science and Engineering,Nankai University,Tianjin 300071,China)

机构地区：[1]天津市城市生态环境修复与污染防治重点实验室环境污染过程与基准教育部重点实验室南开大学环境科学与工程学院

出　　处：《应用与环境生物学报》2018年第4期915-920,共6页Chinese Journal of Applied and Environmental Biology

基　　金：国家重点基础研究发展计划(2015CB459000)资助~~

摘　　要：为去除环境中BDE-47的残留,通过以BDE-47为碳源的选择性培养基驯化,从电子垃圾拆解厂的土壤中分离出了1株厌氧降解BDE-47的纯菌种,命名为XM,并研究其对BDE-47的降解特性.经16S rDNA鉴定,XM属于兼性肠杆菌（Enterobacter sp.）,当BDE-47浓度为525μg/L,初始接菌量为7.1×10^5 cells/mL时,培养35 d后降解率为35.8%,降解产物中检测到BDE-28.BDE-47的降解反应符合一级动力学,拟合结果为ln Ct=-0.104t＋6.22.选择以铁离子、硝酸根和硫酸根作为降解过程中外加的电子受体,BDE-47的降解率明显提高,分别为49.8%、59.1%和67.3%.以上研究结果表明,菌株XM能够有效地降解BDE-47,在电子垃圾污染的生物修复方面具有较好的参考和应用价值.In an effort to remove BDE-47 residues from the environment, a bacterial strain that is capable of utilizing BDE-47 as the sole carbon source was isolated and screened from soil collected from an e-waste recycling area in Tianjin to analyze the degradation properties. The strain was preliminarily identified as Enterobacter sp. according to a 16 S r DNA gene sequence analysis. The strain degraded 35.8% of 525 μg/L of BDE-47 in 35 d when the initial concentration of bacteria was 7.1 × 10^5 cells/mL. The product of the biodegradation of BDE-47 was BDE-28. The biodegradation of BDE-47 fit well with first-order kinetics, and its degradation kinetics was ln Ct =-0.104 t ＋ 6.22. With the addition of an electron acceptor, such as Fe^3＋, SO4^2-and NO3^-, the BDE-47 degradation rate was significantly increased to 49.8%, 59.1%, and 67.3%, respectively. The above results revealed that the strain could degrade BDE-47, which is of importance in the application of environmental bioremediation of BDE-47.

关 键 词：BDE-47 降解菌 筛选鉴定 降解率 电子受体 

分 类 号：X172[环境科学与工程—环境科学]