臭氧和干旱交互作用对杨树叶片抗氧化酶活性的影响  

Interactive Effects of Ozone and Drought on Antioxidant Enzyme Activities of Poplar Leaves

作　　者：周慧敏 李品 高峰 冯兆忠[2,3] 张殷波 ZHOU Hui-min;LI Pin;GAO Feng;FENG Zhao-zhong;ZHANG Yin-bo(Institute of Loess Plateau,Shanxi University,Taiyuan 030006,China;State Key Laboratory of Urban and Regional Ecology,Researeh Center for Eeo-Environmental Seienees,Chinese Academy of Seienees,Beijing 100085,China;College of Resources and Environment,University of Chinese Academy of Sciences,Beijing 101408,China;College of Environmental Seienee and Resources,Shanxi University,Taiyuan 030006,China)

机构地区：[1]山西大学黄土高原研究所,太原030006 [2]中国科学院生态环境研究中心,城市与区域生态国家重点实验室,北京100085 [3]中国科学院大学资源环境学院,北京101408 [4]山西大学环境与资源学院,太原030006

出　　处：《环境科学》2018年第9期4359-4365,共7页Environmental Science

基　　金：国家自然科学基金项目(31500396,41771034)

摘　　要：地表臭氧（O3）污染和干旱成为严重威胁植物正常生长的主要环境胁迫因子.本文研究3种O3浓度（CF：过滤空气O3;NF：未经过滤的环境空气;NF40：NF＋40 nmol·mol-1O3）和2种水分处理（充分灌溉;干旱,60%灌溉）及其交互作用对敏感性杨树品种‘546’（Populus deltoids cv.55/56×P.deltoides cv.Imperial）叶片饱和光合速率、抗氧化酶活性及可溶性蛋白含量的影响.结果表明,O3浓度升高显著降低饱和光合速率,干旱处理对饱和光合速率的影响不显著,O3和干旱的交互作用显著.干旱显著降低过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性,且随着处理时间的延长CAT和SOD活性降低显著.处理时间的延长也显著降低抗坏血酸过氧化物酶（APX）活性,增加了可溶性蛋白含量.过氧化物酶（POD）不受O3、干旱及O3与干旱交互作用的显著影响.O3处理以及O3与干旱的交互作用均未对抗氧化酶活性产生显著影响.该研究结果为应对环境变化背景下O3浓度升高与干旱加剧对杨树的伤害提供科学依据.Ground-level ozone（O3） pollution and drought have become major environmental factors that threaten the normal growth of plants. In this study,we determined the effects of three O3 concentrations（charcoal-filtered air,CF,non-filtered air,NF and nonfiltered air ＋ 40 nmol·mol-1,NF40）,two water treatments（well-watered,WW,and mild drought,MD,60% of WW in volumetric soil water content）,and their interactions on the light-saturated photosynthesis rate（Asat） and on the activity of antioxidant enzymes and soluble proteins of the poplar ‘546 ＇（Populus deltoids cv. 55/56 × P. deltoides cv. Imperial）. Results showed that Asat significantly decreased with elevated O3,and with interactions between O3 and drought. Drought significantly reduced the activities of catalase（CAT） and superoxide dismutase（SOD） but did not affect the activities of peroxidase（POD） or ascorbate peroxidase（APX）. Reduction ranges in CAT and SOD increased with drought duration. APX activity significantly decreased with increasing O3 and drought,but soluble protein content did not. Activities of antioxidant enzymes were not significantly affected by elevated O3 and their interactions. Results provide a scientific basis for protection of poplar plantations in response to increasing O3 concentrations and drought under environmental changes.

关 键 词：杨树 臭氧 干旱 交互 抗氧化酶 

分 类 号：X171.5[环境科学与工程—环境科学]