白花蛇舌草提取物对乳腺癌MCF-7细胞增殖和凋亡影响的实验研究  

An Experimental Study on the Effects of Hedyotis Diffusa Extracts on the Proliferation and Apoptosis of MCF-7 Breast Cancer Cells

作　　者：王宇[1] 宋舟[1] 康爱文 赵蒙[3] 田丽媛[4] WANG Yu;SONG Zhou;KANG Ai-wen;ZHAO Meng;TIAN Li-yuan(Department of general surgery,PLA General Hospital,Beijing,100853,China;Department of general surgery,Weichang County Hospital,Chengde,Hebei,068450,China;Department of Gastrointestinal Surgery,Heze Municipal hospital,Heze,Heze,274031,China;Department of clinical laboratory,PLA General Hospital Hainan Branch,Sanya,Hainan,572013,China)

机构地区：[1]解放军总医院普通外科,北京100853 [2]河北省围场县医院普通外科,河北承德068450 [3]山东省菏泽市立医院胃肠外科,山东菏泽274031 [4]解放军总医院海南分院临床检验科,海南三亚572013

出　　处：《现代生物医学进展》2018年第16期3007-3011,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金青年基金项目(81201336);三亚市医疗卫生科技创新项目(2015YW21)

摘　　要：目的:探索不同浓度的白花蛇舌草提取物对人乳腺癌MCF-7细胞的增殖和凋亡的影响。方法:选择体外培养的人乳腺癌MCF-7细胞,将其分为5组,分别给予不同浓度使用乙醇提取法提取的白花蛇舌草有效成分(0、75、150、300和600μg/mL)。分别在药物孵育的第1、2、3、4天提取细胞样本,采用MTT法检测细胞的增殖情况,流式细胞技术检测细胞周期和细胞凋亡情况。结果:在第1、2、3和4天,不同浓度的白花蛇舌草均能够显著抑制乳腺癌细胞的增殖,且细胞的增殖抑制率随着药物浓度的升高而升高,以300μg/mL和600μg/mL两个浓度的作用效果最为显著,但300μg/mL和600μg/mL两个浓度间无统计学差异(P>0.05)。不同浓度的白花蛇舌草干预后处于G0~G1期的细胞百分比显著低于对照组(P<0.05),处于S期和G2~M期的细胞百分比高于对照组(P<0.05),且细胞凋亡率显著高于对照组(P<0.05),对细胞周期的调控和凋亡的抑制效果以300μg/mL和600μg/mL两个浓度最为显著,但两个浓度间无统计学差异(P>0.05)。结论:白花蛇舌草能够显著抑制乳腺癌MCF-7细胞的增殖并促进细胞凋亡,且这一作用存在显著的"量-效"关系。Objective： To explore the effects of various concentrations of hedyotis diffusa extracts on the proliferation and apoptosis of MCF-7 human breast cancer cells. Methods： The MCF-7 human breast cancer cells were cultured in the DMEM medium with 10% fetal bovine serum,1% penicillin/streptomycin and insulin. The active components of hedyotis diffusa were extracted using the ethanol-extracting method. Overall 5 groups were included in the study,including four hedyotis diffusa administration groups with different concentrations（75,150,300 and 600 μg/mL） and the blank control group. Results： The cell samples were collected at 1,2,3,and 4 days post the drug application,and the effects of hedyotis diffusa on cellular proliferation rate were evaluated the MTT and CCK-8 methods,respectively. Then,the efficacy of hedyotis diffusa with different concentrations（75,150,300 and 600 μg/mL） on the cell cycle and cell apoptosis was investigated based on the flow cytometry technique. Conclusion： Hedyotis diffusa could significantly inhibit the proliferation and promote the apoptosis of breast cancer cells in a concentration dependent manner.

关 键 词：白花蛇舌草 乳腺癌 细胞增殖 MTT检测 细胞凋亡 

分 类 号：R-33[医药卫生] R737.9