构成单元的多肽构象对融合肽功能的影响  

Effect of peptide conformation of constituent unit on function of chimeric peptide

作　　者：赵敏[1] 毛靖[1] 王丹[1] ZHAO Min;MAO Jing;WANG Dan(Department of Stomatology,Tongfi Hospital,Tongji Medical College,Huazhong University of Science and Tech-nology,Hubei Wuhan 430030,China)

机构地区：[1]华中科技大学同济医学院附属同济医院口腔医学中心,湖北武汉430030

出　　处：《临床口腔医学杂志》2018年第8期464-467,共4页Journal of Clinical Stomatology

基　　金：国家自然科学基金(81301327)

摘　　要：目的:比较三种具有不同多肽构象的融合肽对金属钛表面的亲和性及其对轻力负载下细胞反应的双向生物学功能。方法:设计3种不同多肽构象的融合肽:TBP-RGD,TBP-cyclicRGD(含有环状结构RGD)和TBP-linerRGD(含有线性结构RGD);利用石英晶体微天平(QCM-D)比较3种融合肽在钛表面的结合能力;通过MC3T3-E1成骨细胞黏附、增殖实验比较了融合肽对离心力负载下细胞的生物反应。结果:QCM-D显示TBP-cyclicRGD与金属钛表面的亲和性最强,而TBP-linerRGD最弱;经过融合肽涂层修饰的钛表面,48 h时TBP-cyclicRGD促细胞增殖最强。结论:构成单元中不同多肽构象的融合肽对钛表面的结合力有差异,其中环状构象的TBP-cyclicRGD能显著提高融合肽对金属钛的识别和结合能力,并有助于提高成骨细胞在力学负载条件下的增殖活动。Objective： The present study aimed to compare the affinity of three chimeric peptides with different peptide conformation of chain structure units to titanium（ Ti） surface and their bidirectional biological functions in promoting cellular response to mechanical loading. Methods： Three chimeric peptides were designed,TBP-RGD,TBP-cyclicRGD and TBP-linerRGD. A quartz crystal microbalance（ QCM-D） was used to detect the affinities of three chimeric peptides on titanium surface. Cell adhesion and proliferation of MC3 T3-E1 osteoblasts on chimeric peptide pre-coating Ti disks were compared after mechanical centrifugal loading. Results： TBP-cyclicRGD had the strongest affinity to titanium surface,while TBP-linerRGD had the weakest affinity. After 48 hours,cells on TBP-cyclicRGD precoating Ti proliferated most comparing with other groups. Conclusion： The affinities of three chimeric peptides to Ti surface varied with different peptide conformation. TBP-cyclicRGD performed the best recognition and binding ability to Ti,and it also helped to improve the osteoblast proliferation under mechanical loading.

关 键 词：融合肽 钛 成骨细胞 石英晶体微天平(QCM-D) 力加载 

分 类 号：R783.1[医药卫生—口腔医学]