牛磺酸对锰染毒大鼠纹状体神经组织细胞细胞周期及凋亡的影响  

Effect of the taurine on striatum tissue cell cycle and apoptosis in manganese exposed rats

作　　者：吴松林 秦绚[2] 郭松超[3] 

机构地区：[1]厦门医学院预防医学教研室,361023 [2]山西省中医院 [3]广西医科大学公共卫生学院

出　　处：《中华劳动卫生职业病杂志》2018年第8期568-572,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目（30260095）

摘　　要：目的观察细胞周期和细胞凋亡的变化，探讨牛磺酸（Tau）对锰染毒大鼠神经毒性的影响。方法取SD健康雄性大鼠156只，随机分为对照组，10、15、20mg／kg3个剂量的锰染毒组和Tau干预组（100、150、200mg／kgTau），每组12只大鼠。干预12周后，开颅分离出大鼠纹状体，分别进行Tunel染色、锰含量水平测定、流式细胞仪测定细胞周期。结果锰染毒组和Tau干预组纹状体细胞凋亡指数均高于对照组，差异有统计学意义（P〈0．05）。150、200mg／kgTau干预对10、15、20mg／kg锰染毒组大鼠纹状体细胞凋亡指数有降低作用，差异有统计学意义（P〈0．05）；各染锰组和Tau干预组纹状体锰含量均高于对照组，差异均有统计学意义（均P〈O．05）；锰染毒组G0／G1期细胞所占的比例均比空白对照组低，S期细胞比例均比空白对照组高，差异均有统计学意义（均P〈0．05）。结论锰可将大鼠纹状体神经组织细胞的细胞周期阻滞于S期，致大鼠纹状体神经组织细胞出现程度不同的细胞凋亡，牛磺酸在一定程度上对锰诱导的大鼠纹状体神经组织细胞凋亡有保护作用。Objective To elucidate the effect of taurine on neurotoxicity induced by Mn by investigating cell cycle and apoptosis in manganese exposed rats. Methods 156 healthy male SD rats were randomly divided into 1 control group, 3 manganese exposure groups （10, 15, and 20 mg/kg respectively）, and 9 intervened groups based on orthogonal design, with 12 rats in each group. After 12 weeks of exposure, all rats were decapitated and striatums were removed, cell cycle was analyzed by flow cytometry, the apoptosis was detected by TUNEL, level of Mn was determined. Results The striatum apoptosis index of the 3 dose groups exposed to Mn were significantly higher than control group（P〈0.05 ）. The striatum apoptosis index of the 9 intervened groups were significantly higher than control group （P〈0.05）. 150 and 200 mg/kg of taurine could decrease apoptosis index of the group exposed to 10,15,20 mg/kg of Mn （P〈0.05）. The striatum Mn content of the 3 dose groups exposed to Mn were significantly higher than that of the control group （P〈0.05）. The G0/G1 proportion of the 3 dose groups exposed to Mn were significantly lower than that of the control group （P〈0.05）, the S proportion of the 3 dose groups exposed to Mn were significantly higher than that of the control group （P〈 0.05）. Conclusion Mn could cause cell cycle arrest to S , increase level of apoptosis in striatum, to a certain extent, taurine can protect neurons from apoptosis induced by Mn.

关 键 词：牛磺酸 锰 细胞周期 细胞凋亡 大鼠 

分 类 号：R114[医药卫生—卫生毒理学]