3种方法提取沙枣黄酮工艺的优化研究  

Study on Optimization of Extraction Technology of Flavonoids from Elaeagnus angustifolia L. by Three Methods

作　　者：王楠 刘战霞 程卫东[1] WANG Nan;LIU Zhan-xia;CHENG Wei-dong(College of Food Science,Shihezi University,Shihezi,Xinjiang 83200)

机构地区：[1]石河子大学食品学院,新疆石河子832000

出　　处：《安徽农业科学》2018年第26期150-155,共6页Journal of Anhui Agricultural Sciences

摘　　要：[目的]对比分析几种提取沙枣黄酮的方法,优化沙枣黄酮的提取工艺条件。[方法]以沙枣为研究对象,沙枣黄酮为目标提取物,通过单因素和响应面试验分析对比3种提取沙枣黄酮的方法,以获得提取沙枣黄酮的最优工艺条件。[结果]超声波辅助提取沙枣黄酮效果最好,微波辅助提取法次之,传统溶剂提取法提取率最低。超声波辅助提取法最优工艺条件为提取温度62.02℃,料液比1∶15.65(g∶m L),乙醇浓度70.32%时,沙枣黄酮含量11.320 mg/g。[结论]该研究可为沙枣黄酮纯化及应用试验提供参考依据。[Objective]To optimize the extraction conditions of flavonoids from Elaeagnus angustifolia L. [Method] Elaeagnus angustifolia L. was used as the research object,the flavonoids of Elaeagnus angustifolia L. were the target extract. 3 methods of extracting flavonoids from Elaeagnus angustifolia L. were analyzed and compared by single factor and response surface test, so as to obtain the optimum technological conditions for extracting flavonoids from Elaeagnus angustifolia L.. [Result]The results indicated that the ultrasonic assisted extraction was the best, followed by microwave-assisted extraction, and the traditional solvent extraction was the lowest. The optimum conditions of ultrasonic assisted extraction are as follows： the extraction temperature is 62.02 ℃, the ratio of material to liquid is 1 ∶15.65（g ∶mL）, and the concentration of ethanol is 70.32%, the content of flavonoids in Elaeagnus elaanus is 11.320 mg/g. [Conclusion]The study can provide reference for the purification and application of flavonoids from Elaeagnus angustifolia L.

关 键 词：沙枣 黄酮 提取方法 响应面 工艺优化 

分 类 号：TS201.2[轻工技术与工程—食品科学]