三氧化二砷对膀胱癌T24细胞增殖及APC基因表达的影响  

Effect of arsenic trioxide on the proliferation and gene expression of APC in bladder cancer T24 cells

作　　者：孙军培 刘久华[2] 杜丹丽[1] 杨晓东 张燕[1] SUN Jun-pei;LIU Jiu-hua;DU Dan-li;YANG Xiao-dong;ZHANG Yan(Reprodwc.tive Center,TIw First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004;Department of Urology,TIw Eastern Hospital of the Second People＇s Hospital of Lianyungang Affiliated to Bengbu Medical College,Lianyungang Jiangsu 222006,China)

机构地区：[1]蚌埠医学院第一附属医院生殖医学中心,安徽蚌埠233004 [2]蚌埠医学院附属连云港市第二人民医院东院区泌尿外科,江苏连云港222006

出　　处：《蚌埠医学院学报》2018年第8期989-992,共4页Journal of Bengbu Medical College

摘　　要：目的:探讨三氧化二砷(arsenic trioxide,As_2O_3)对人膀胱癌T24细胞增殖及腺瘤性结肠息肉病相关基因(adenomatous polyposis coli,APC)表达的影响。方法:采用2、4、8μmol/L的As_2O_3处理T24细胞,MTT法检测T24细胞增殖抑制率;脱氧核糖核酸原位末端转移酶标记技术检测细胞凋亡;用RT-PCR及Western blot法分别检测As_2O_3对T24细胞中APC mRNA和蛋白表达的影响。结果:As_2O_3在2~8μmol/L浓度范围内处理T24细胞72 h可有效抑制细胞增殖(P<0.01);促进细胞凋亡(P<0.01);增加APC mRNA和蛋白表达(P<0.01)。结论:在一定浓度范围内(2~8μmol/L)As_2O_3可以有效抑制T24细胞增殖和诱导其凋亡,其机制可能与上调APC基因的表达有关。Objective： To observe the effects of arsenic trioxide（ As2O3） on the proliferation and gene expression of adenomatous polyposis coli（ APC） in human bladder cancer T24 cells. Methods： The T24 cells were processed with 2,4 and 8 μmol/L of As2O3,the proliferation inhibition rate was detected using MTT assay,the apoptosis was detected using the deoxyribose situ terminal transferase labeling,and the expression levels of APC mRNA and protein were detected using RT-PCR and Western blot,respectively. Results： The2 to 8 μmol/L of As2O3 treated T24 cells for 72 h could effectively inhibit the cell proliferation（ P〈0. 01）,promote the cell apoptosis（ P〈0. 01）,and increase the expression of APC mRNA and protein（ P〈0. 01）. Conclusions： As2O3（ 2 - 8 μmol/L） can effectively inhibit the proliferation,and induce the apoptosis of T24 cells,the mechanism of which may be related to the upregulation of APC gene expression.

关 键 词：膀胱肿瘤 腺瘤性结肠息肉病相关基因 三氧化二砷 细胞凋亡 

分 类 号：R737.14[医药卫生—肿瘤]