白花蛇舌草提取物对乳腺癌荷瘤小鼠干预作用的实验研究  

An Experimental Study on the Therapeutic Effects of Hedyotis Diffusa Extracts on the Breast Cancer in Tumor-bearing Mice

作　　者：宋舟[1] 王宇[1] 卢灿荣[1] 卫勃[1] 陈凛[1] SONG Zhou;WANG Yu;LU Can-tong;WEI Bo;CHEN Lin(Department of general surgery,PLA General Hospital,Beijing,100853,China)

机构地区：[1]解放军总医院普通外科,北京100853

出　　处：《现代生物医学进展》2018年第15期2801-2805,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81602507;81773135)

摘　　要：目的:明确白花蛇舌草提取物对乳腺癌荷瘤小鼠肿瘤发生发展的干预作用及可能机制,以期为其未来的临床应用提供实验依据。方法:选择12周龄的雄性BALB/c小鼠30只,随机等分成3组:对照组(Control)、乳腺癌荷瘤小鼠组(BC)和乳腺癌荷瘤小鼠行白花蛇舌草药物治疗组(BC+HD)。BC组和BC+HD组小鼠的右侧肩胛骨注射以0.2 m L的MCF-7乳腺癌细胞悬液(细胞浓度为2×10~7/m L),Control组小鼠注射等量的生理盐水。在2周后,对BC+HD组小鼠进行白花蛇舌草的灌胃给药,BC组和Control组小鼠灌以等量的生理盐水。在给药的第21天后,处死全部小鼠,分析小鼠的体重、总摄食量、肿瘤重量和肿瘤体积,使用酶联免疫吸附测定(enzyme linked immunosorbent assay,ELISA)法定量分析小鼠血清中的白介素-6(interleukelin-6,IL-6)和血管内皮生长因子(vascular endothelial growth factor,VEGF)的含量。结果:给药21天后,BC+HD组小鼠体重显著高于BC组(P<0.05),总摄食量、肿瘤重量、肿瘤体积、血清IL-6和VEGF的含量均显著低于BC组(P<0.05)。结论:白花蛇舌草能够有效改善乳腺癌荷瘤小鼠的营养状况,抑制乳腺癌的发生和发展,可能与其有效降低乳腺荷瘤小鼠血清IL-6和VEGF的含量有关。Objective： To identify the effects and potential mechanisms of hedyotis diffusa extracts on the occurrence and development of breast cancer in tumor-bearing mice, and thus provide experimental evidences for the following clinical application of hedyotis diffusa. Methods： Twelve-week-old male BALB/c mice（n=30） were randomly and equally assigned into 3 groups, including the blank control（Control）, breast cancer（BC） and breast cancer with hedyotis diffusa treatment（BC＋HD） groups. The mice in the BC and BC＋HD groups were subjected to injection of MCF-7 cell suspension（0.2 m L, 2×10~7/m L） from the right scapula. The mice in the Control group were injected with saline with the same volume. After two weeks, the mice in the BC＋HD groups received hedyotis diffusa administration by gavage, the mice in the BC and Control groups received saline with the same volume. Twenty one days post hedyotis diffusa administration, all mice in the three groups were sacrificed, and the body weight, total feed volume, tumor weight and tumor volume were analyzed and quantified. The serum interleukelin-6（IL-6） and vascular endothelial growth factor（VEGF） concentrations were also determined using the enzyme linked immunosorbent assay（ELISA） method. Results： At 21 days after treatment, the body weight of mice in the BC＋HD group were significantly higher than those in the BC group（P〈0.05）, and the total feed volume, tumor weight, tumor volume, serum IL-6 and serum VEGF concentrations in the BC＋HD group were significantly lower than those in the BC group（P〈0.05）.Conclusion： Hedyotis diffusa could significantly improve the nutritional status and inhibit the occurrence and development of breast cancer. This efficacy of hedyotis diffusa might be associated with the significant reduction of inflammation-related cytokine expression.

关 键 词：白花蛇舌草 乳腺癌 荷瘤小鼠 肿瘤体积 

分 类 号：R-33[医药卫生] R737.9