GINS2过表达对NB4细胞和K562细胞凋亡的影响  

Effect of Overexpression of GINS2 on Apoptosis of NB4 Cells and K562 Cells

作　　者：高艳军[1] 邢志伟[1] 刘相[1] 李刚[1] 张建武[1] 王世博[2] GAO Yanjun;XING Zhiwei;LIU Xiang(Hebei Chest Hospital,Heibei Shijiazhuang 050041,China)

机构地区：[1]河北省胸科医院检验科,河北石家庄050041 [2]河北医科大学第二医院感染控制处,河北石家庄050000

出　　处：《河北医学》2018年第9期1413-1416,共4页Hebei Medicine

基　　金：河北省医学科学研究重点课题计划项目;(编号:20150602)

摘　　要：目的:利用Ad-GINS2感染白血病细胞NB4细胞和K562细胞,观察其对白血病细胞凋亡的影响。方法:通过Ad-GINS2感染NB4细胞和K562细胞,荧光显微镜观察荧光表达情况,流式细胞术检测K562细胞和NB4细胞的凋亡和细胞周期分布情况,利用Western blotting技术检测凋亡蛋白bax和抗凋亡蛋白bcl-2的表达水平。结果:Ad-GINS2感染NB4细胞和K562细胞,72h后观察荧光表达情况;流式细胞术检测NB4细胞和K562细胞的凋亡率下降,与空载组和未处理组相比,差别有统计学意义(P<0.05);细胞周期分布结果显示,NB4细胞S期比例升高,G1细胞比例下降,与未处理组及空载组相比,差别有统计学意义(P<0.05);K562细胞S期和G2期细胞比例升高,G1细胞比例下降,与未处理组及空载组相比,差别有统计学意义(P<0.05);Western blotting技术检测凋亡蛋白bax下降,抗凋亡蛋白bcl-2表达升高。结论:GINS2基因的重组腺病毒载体成功感染NB4细胞和K562细胞并且抑制细胞凋亡,进而发现细胞凋亡可能与细胞周期进程相关,为进一步明确GINS2基因在白血病中的作用奠定了一定基础。Objective： To observe its effect on leukemia cell apoptosis by using Ad-GINS2 infection.Methods： NB4 cells and K562 cells were infected by Ad-GINS2 and using fluorescence microscope observe the infection effect of NB4 cells and K562 cells; Apoptosis and cell cycle distribution of NB4 cells and K562 cells were detected by FCM; Bax and bcl-2 protein were detected by Western blotting test. Results： Fluorescence were observed by fluorescence microscope after 72 h; Apoptosis rate was decreased significantly using FCM technology compared with the untreated group and no-load group（ P〈0.05）; Results of cell cycle distribution showed that S,phase rate were increased and G1 phase rate were decreased in NB4 cells compared with the untreated group and no-load group（ P〈0.05）; S G2 phase rate was increased and G1 phase rate were decreased in K562 cells compared with the untreated group and no-load group（ P〈0.05）; Bax protein was decreased and bcl-2 protein was increased detected by Western blotting test. Conclusion： The recombinant adenovirus vector of GINS2 gene successfully infect NB4 and K562 cells and inhibit cell apoptosis,and then found that apoptosis may be related to the process of cell cycle,which lays a foundation for further clarifying the role of GINS2 gene white in leukemia.

关 键 词：Ad-GINS2 NB4细胞 K562细胞 过表达 凋亡 

分 类 号：R733.7[医药卫生—肿瘤]