干扰IL-8表达对乳腺癌细胞侵袭、迁移能力的影响  

Effects of interfering the expression of IL-8 on biological characteristics of breast cancer cells

作　　者：牟成金 潘武 李娟 汪静[2] MOU Chengjin;PAN Wu;LI Juan;WANG Jing(Sichuan Academy of Medical Sciences,Sichuan Provincial People＇s Hospital（East Hospital）,Chengdu 610101,China;Department of breast surgery,West China Hospital,Sichuan University,Chengdu 610041,China)

机构地区：[1]四川省医学科学院.四川省人民医院甲状腺乳腺外科,成都610101 [2]四川大学华西医院乳腺外科,成都610041

出　　处：《免疫学杂志》2018年第9期765-771,共7页Immunological Journal

基　　金：四川省科技厅科研项目(2015SZ0070)

摘　　要：目的探究IL-8对乳腺癌MDA-MB-231细胞生物学特性的影响以及作用机制。方法将siRNA IL-8转染至乳腺癌细胞中,实验分为空白对照组、阴性对照组、siRNA1 IL-8组、siRNA2 IL-8组。噻唑蓝(MTT)实验观察干扰IL-8基因表达对细胞活力的影响,Transwell小室法检测细胞侵袭、迁移能力的变化,采用荧光定量PCR(RT-PCR)和酶联免疫吸附测定法(ELISA)检测转染后IL-8 mRNA和蛋白水平,蛋白质印迹法(Western blot)法检测Wnt/β-catenin信号通路的变化。结果与空白对照组相比,siRNA1 IL-8组、siRNA2 IL-8组乳腺癌细胞中IL-8 mRNA和蛋白表达量显著下降(P<0.05);siRNA2 IL-8组细胞中IL-8 mRNA和蛋白水平较siRNA1 IL-8组显著下调(P<0.05),表明siRNA2 IL-8的干扰效果较强,因此用于后续实验。干扰IL-8表达对抑制乳腺癌细胞的活力无显著影响,抑制其侵袭、迁移能力(P<0.05),细胞中β-catenin、MMP-2、MMP-9的蛋白水平显著降低(P<0.05)。结论干扰IL-8表达可能通过抑制Wnt/β-catenin信号通路从而抑制乳腺癌MDA-MB-231细胞侵袭、迁移能力。Interleukin-8（IL-8）was closely related to the occurrence and development of tumors,but its specific role in breast cancer cells is not fully understood. To investigate the effects of interleukin-8（IL-8）on the biological characteristics of breast cancer MDA-MB-231 cells and its mechanism. The siRNA IL-8 was transfected into breast cancer cells. The experiment were divided into blank control group,negative control group,siRNA1 IL-8 group,siRNA2 IL-8 group. The viability ofbreast cancer cells was measured by MTT assay. The invasion and migrationwere analyzed bytranswell assay. The levels of IL-8 were examined by real-time RT-PCR and ELISA. The activity of Wnt/β-catenin signal pathway were detected by Western blot. Compared with the blankcontrol group,the expression of IL-8 mRNA and protein in breast cancer cells in group siRNA1 IL-8 and siRNA2 IL-8 were decreased significantly（P〈0.05）. The levels of IL-8 mRNA and protein in siRNA2 IL-8 group were significantly lower than those in siRNA1 IL-8 group（P〈0.05）,indicating that siRNA2 IL-8 had strong interference effect,so it will be used for the subsequent experiments. The expression of interfering the expression of IL-8 had no significant effect on the viability of breast cancer cells,but inhibited invasion and migration ability of breast cancer cells（P〈0.05）,reduced the protein levels of β-catenin,MMP-2,MMP-9（P〈0.05）. Interfering the expression of IL-8 may inhibitthe invasion and migration of breast cancer MDAMB-231 cells by inhibiting the Wnt/β-catenin signaling pathway.

关 键 词：IL-8 侵袭 乳腺癌 WNT/Β-CATENIN 

分 类 号：R737.9[医药卫生—肿瘤]