1-甲基色氨酸对卵巢癌细胞耐药性的逆转  

Inversion of drug resistance in ovarian cancer by 1-methyl tryptophan

作　　者：冯勤梅[1] 侯茜媛 吕真娇 白宁 Feng Qinmei;Hou Qianyuan;Lyu Zhenjiao;Bai Ning(Department of Ggynaecolog;Shanxi Provincial People＇s Hospital,Taiyuan 030012,China)

机构地区：[1]山西省人民医院妇科,太原030012 [2]山西医科大学第一医院妇科

出　　处：《中国药物与临床》2018年第9期1465-1467,共3页Chinese Remedies & Clinics

基　　金：山西省自然科学基金(201701D121156)

摘　　要：目的通过2,3-双加氧酶(IDO)抑制剂1-甲基色氨酸(MT-1)对耐药细胞的生物学特性监测,探讨MT-1是否可以逆转耐药卵巢癌细胞的耐药性。方法将MT-1与耐药卵巢癌细胞株即SKOV3/CBP细胞株共培养后,用酶联免疫法检测细胞内IDO浓度,MTT法监测细胞耐药及细胞增殖速度,Matrigel小室检测细胞侵袭能力。结果与未加入MT-1组相比,加入MT-1组SKOV3/CBP中IDO的表达明显降低且差异有统计学意义(P<0.05);同时加入MT-1组SKOV3/CBP细胞的半数抑制浓度(IC50)及耐药指数(RI)均明显下降,且癌细胞的侵袭能力降低均较未加入组差异有统计学意义(P<0.05),加入MT-1后对耐药细胞的增殖能力增加但较未加入组在各时间段差异无统计学意义(P>0.05)。结论 MT-1降低耐药SKOV3/CBP中IDO的表达,降低耐药细胞的半增殖指数及细胞的侵袭能力从而逆转其耐药性,MT-1可作为卵巢癌耐药治疗的新手段。Objective To monitor the biological characteristics of drug resistant cells by the IDO inhibitor 1- methyl tryptophan （MT-1）, and to investigate whether MT-1 can inverse drug resistance in resistant ovarian cancer cells. Methods After co-culture of MT-1 with the resistant ovarian cancer cell line SKOV3/CBP, the intracellular IDO concentration was determined by enzyme-linked immunosorbent assay. MTT method was used to monitor the drug resistance and proliferation rate of cells, and Matrigel chamber was used to determine the cell invasion ability-. Re- suits Compared with the SKOV3/CBP cell line group, the expression of IDO in the SKOV3/CBP cell line＋MT-1 group significantly decreased, with statistically significant difference （P〈0.05）. At the same time, the inhibitory con- eentration （IC50） and resistance index （RI） in the SKOV3/CBP cell line＋MT-1 group significantly decreased, and the invasiveness of cancer cells decreased compared with that in the SKOV3/CBP cell line group （P〈0.05）. The proliferation ability of drug-resistant cells increased in the SKOV3/CBP cell line＋MT-1 group, but there was no signifi- cant difference compared with the SKOV3/CBP cell line group at different time-points （P〉0.05）. Conclusion MT-1 can decrease the expression of IDO in drug-resistant SKOV3/CBP cells,the semi-proliferation index and invasiveness of drug-resistant cells, thus inversing the drug resistance. MT-1 can be used as a novel drug therapy for ovarian cancer.

关 键 词：卵巢肿瘤 卡铂 抗药性 肿瘤 1-甲基色氨酸 

分 类 号：R737.31[医药卫生—肿瘤]