2种实验性增生性玻璃体视网膜病变造模方法比较研究  

Comparison of two methods for modeling proliferative vitreoretinopathy

作　　者：刘丽娅[1] 马景学[1] 安建斌[1] 马月磊[1] LIU Li-ya;MA Jing-xue;AN Jian-bin;MA Yue-lei(Department of Ophthalmology,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,China)

机构地区：[1]河北医科大学第二医院眼科,河北石家庄050000

出　　处：《河北医科大学学报》2018年第10期1201-1204,1209,共5页Journal of Hebei Medical University

基　　金：河北省医学科学研究重点课题(20170074)

摘　　要：目的采用2种方法进行兔眼内增生性玻璃体视网膜病变(proliferative vitreoretinopathy,PVR)模型的建立,对比2种方法的成模时间和效果。方法两实验组分别给予玻璃体腔注射视网膜色素上皮(retinal pigment epithelium,RPE)细胞与白细胞介素1β(interleukin-1β,IL-1β)的混合液或单纯RPE细胞进行造模,对照组给予玻璃体腔注射生理盐水,术后进行玻璃体、眼底、B超等检查,对玻璃体混浊程度和PVR程度进行分级,并计算视网膜脱离发生率。结果术后7d2组PVR发生率均为100%,但注射RPE细胞联合IL-1β组较单纯注射RPE细胞组PVR发生更早、程度更重、视网膜脱离发生率更高。结论通过玻璃体腔注射RPE细胞联合IL-1β建立兔眼PVR模型的方法较单纯注射RPE细胞效果更佳,更符合PVR的病理过程。Objective To compare the time and effect of the 2 methods by using 2 methods to establish proliferative vitreoretinopathy（PVR） model in rabbit eye.Methods The experimental group was given intravitreal injection of a mixture of retinal pigment epithelium（RPE）cells and interleukin-1（IL-1）beta or simple RPE cells respectively.The control group was given intravitreal injection of normal saline,and vitreous,fundus and B ultrasonic examination were performed after operation.The degree of vitreous opacity and the degree of PVR were graded,and the incidence of retinal detachment was calculated.Results After 7 days of vitreous injection,occurrences of PVR were 100%in both of the two groups.PVR happened more earlier and faster in Group Ⅰ than Group Ⅱ.Group Ⅰ also had the higher incidence of retinal detachment.Conclusion Injective vitreous of RPE cells combined with IL-1βin rabbit eyes is the best method to establish of PVR model,and is more in line with the pathological process of PVR.

关 键 词：玻璃体视网膜病 增生性 视网膜色素上皮 视网膜脱离 

分 类 号：R774.1[医药卫生—眼科]