反硝化功能基因nirS和nirK及其检测技术研究进展  

Advances in Denitrification Functional Gene nirS and nirK and Their Detection Technology

作　　者：张旭志[1] 杨倩倩[1,2] 赵俊[1] 曲克明[1] ZHANG Xu-zhi;YANG Qian-qian;ZHAO Jun;QU Ke-ming(Yellow Sea Fisheries Res.Inst.,Chinese Acad.of Fishery Sci.;Qingdao Nat＇l Lab.for Marine Sci.＆ Technol.,Qingdao 266071;2.Coll.of Marine Sci.Shanghai Ocean Uni.,Shanghai 201306)

机构地区：[1]中国水产科学研究院黄海水产研究所青岛海洋科学与技术国家实验室,山东青岛266071 [2]上海海洋大学海洋科学学院,上海201306

出　　处：《微生物学杂志》2018年第4期84-91,共8页Journal of Microbiology

基　　金：黄海水产研究所基本科研项目(20603022016003);中国水产科学研究院基本科研项目(2016RC-BR02);青岛海洋科学与技术国家实验室鳌山科技创新计划项目(2015ASKJ02);青岛海洋科学与技术国家实验室鳌山人才计划

摘　　要：反硝化作用对维持环境氮平衡具有重要意义,其过程中亚硝酸盐向NO的转化是关键步骤,由亚硝酸盐还原酶控制,因此检测亚硝酸盐还原酶编码基因可以从分子生物学角度深入分析微生物种群特性和氮元素的转化规律,进而为评估生态环境健康状况等科研与生产活动提供支撑数据。重点综述了近年来反硝化作用功能基因nirS和nirK及其检测技术的研究进展,并对未来可预期的检测技术进行了展望,以期为该领域的研究人员提供参考。Denitrification has important significance in maintaining environmental balance of nitrogen cycle. During the processes,the transformation of nitrite into nitric oxide is a crux step,catalyzed by nitrite reductase; therefore,the detection of nitrite reductase coding genes could analyze deeply into the specificity of microbial population and the transformation law of the element of nitrogen from the perspective of molecular biology,then proceed to evaluate the healthy condition of eco-environment and the production and living activities to provide supporting data. In this paper advance in denitrification function genes of nir S and nir K,as well as their detection methods were reviewed intensively. And some promising detection methods were also prospected,so as to provide some references to those researchers in the field.

关 键 词：反硝化作用 功能基因 nirS NIRK 检测技术 

分 类 号：Q939.99[生物学—微生物学] X172[环境科学与工程—环境科学]