miR-101调控非小细胞肺癌的发病机制  

The pathogenesis of miR-101 in the regulation of non-small cell lung cancer

作　　者：王海江[1] 宋丽霞[1] 汪文斐[1] 杨争[1] 夏照华[1] 黄丕来 李国保[1] 陈培芬[1] 关彩艳 WANG Haijiang;SONG Lixia;WANG Wenfei;YANG Zheng;XIA Zhaohua;HUANG Pilai;LI Guobao;CHEN Peifen;GUAN Caiyan(Department of Thoracic Surgery,Shenzhen Third People＇s Hospital,Shenzhen 518112,China)

机构地区：[1]深圳市第三人民医院胸外科,广东深圳518112

出　　处：《中国现代医生》2018年第24期8-11,共4页China Modern Doctor

基　　金：广东省医学科学技术研究基金项目(A2016366);广东省深圳市科技计划项目(JCYJ20160427152331946)

摘　　要：目的探讨miR-101在非小细胞肺癌发生、发展中的作用以及新的分子调控机制。方法将miR-101转染到非小细胞肺癌A549细胞株,比较测量人类非小细胞肺癌组织及癌旁组织的miR-101表达水平。采用生物信息学技术预测miR-101在c-Fos的3′UTR潜在结合靶点,然后采用Western blot法和基因敲除方法检测c-Fos的表达和功能。结果分析miR-101在非小细胞肺癌组织及癌旁组织表达,miR-101在非小细胞肺癌组织中低表达(0.00010±0.00002 vs 0.00021±0.00003,P=0.036),过表达miR-101抑制非小细胞肺癌A549细胞增殖并诱导细胞凋亡。并且在非小细胞肺癌细胞中敲除c-Fos和过表达miR-101有相似的效果。结论 miR-101通过c-Fos靶点调节非小细胞肺癌细胞的生长,因此调控miR-101和c-Fos可以应用于非小细胞肺癌潜在的分子治疗。Objective To investigate the role of miR-101 in the development and progression of non-small cell lung cancer and its new molecular regulation mechanism. Methods miR-101 was transfected into non-small cell lung cancer A549 cell line, and the expression level of miR-101 in human non-small cell lung cancer tissues and adjacent tissues was compared. The bioinformatics technique was used to predict the potential binding of miR-101 to the 3＇UTR of c-Fos, and then the expression and function of c-Fos were detected by Western blot and gene knockout. Results The expression of miR-101 in non-small cell lung cancer tissues and adjacent tissues was analyzed, miR-101 was down-regulated in non-small cell lung cancer tissues （0.00010±0.00002 vs 0.00021±0.00003, P=0.036）. And overexpression of miR-101 was inhibited. Overexpression of miR-101 inhibited proliferation and induced apoptosis in non-small cell lung cancer A549 cells. And knocking out c-Fos and overexpressing miR-101 in non-small cell lung cancer cells had similar effects. Conclusion miR-101 regulates the growth of non-small cell lung cancer cells through c-Fos target, so the regulation of miR-101 and c-Fos can be applied to the potential molecular therapy of non-small cell lung cancer.

关 键 词：微小RNA-101 C-FOS 非小细胞肺癌 发病机制 

分 类 号：R734.2[医药卫生—肿瘤]