TGF-β信号通路促进小鼠乳腺癌活检相关性肺转移的实验研究  

Experimental sudy on the TGF-β signaling pathway in promoting mice breast cancer biopsy-related lung metastasis

作　　者：傅永强[1] 郭方明[1] 陈浩浩[1] 傅晓艳[1] 何国产[1] 张辉 方远书 应晓倩[1] 张宁[1] 丁明星[1] FU Yongqiang;GUO Fangming;CHEN Haohao;FU Xiaoyan;HE Guochan;ZHANG Hui;FANG Yuanshu;YING Xiaoqian;ZHANG Ning;DING Mingxing(Jinhua Polytechnic Medical School,Jinhua 321007,China;Jinhua Institute of Food and Drug Test Center in Zhe-jiang Province,Jinhua 321007,China)

机构地区：[1]金华职业技术学院医学院,浙江金华321007 [2]浙江省金华市食品药品检验检测研究院,浙江金华321007

出　　处：《中国现代医生》2018年第23期28-32,35,共6页China Modern Doctor

基　　金：浙江省实验动物科技计划项目(2016C37095);浙江省金华市科技计划项目(2018-3-024)

摘　　要：目的探讨空芯针穿刺活检(core needle biopsy,CNB)对乳腺癌远处转移的影响及其可能机制。方法 30只BALB/c小鼠采用乳腺癌4T1细胞乳腺脂肪垫接种,分成活检与非活检两组,活检组应用CNB建立活检相关肺转移模型,采用流式细胞术、qPCR及形态学等检测技术从肿瘤组织局部和整体水平评价其生物学行为。结果成功建立稳定而可靠的乳腺癌肺转移模型,活检组小鼠肺转移结节数显著高于非活检组(P<0.05);与非活检组相比,活检组小鼠TGF-β1、SOX-4及EZH2的m RNA表达上调(P<0.05),流式细胞术检测的NK细胞增加,但差异无统计学意义(P>0.05)。结论 CNB可通过TGF-β信号通路介导的上皮-间质转化(epithelial-mesenchymal transition,EMT)等机制促进乳腺癌的肺转移。Objective To investigate the effect of core needle biopsy（CNB） on distant metastasis of breast cancer and its possible mechanism. Methods 30 BALB/c mice were inoculated with mammary fat pad of breast cancer 4T1 ceils. They were divided into biopsy group and non-biopsy group. The biopsy group was given CNB to establish a biopsy-related lung metastasis model. Flow eytometry, qPCR, and morphological techniques were used to evaluate the biological be- havior by the local and global levels of tumor tissues. Results After successfully establishing a stable and reliable breast cancer lung metastasis model, the number of lung metastatic nodules in the biopsy group was significantly higher than that in the non-biopsy group（P〈0.05）; Compared with non-biopsy group, mRNA expression of TGF-β1, SOX-4 and EZH2 in the biopsy group was up-regulated（P〈0.05）. NK cells detected by flow eytometry were increased, but the difference was not statistically significant（P〉0.05）. Conclusion CNB can promote lung metastasis of breast cancer through TGF-β signaling pathway-mediated epithelial-mesenehymal transition （EMT） and other mechanisms.

关 键 词：乳腺癌 肺转移 活检 上皮间质转化 小鼠 

分 类 号：R737.9[医药卫生—肿瘤]