利用GC/MS代谢组学技术检测纳米TiO_2对多头绒泡菌代谢途径的扰动作用  

The detection of nano-sized TiO_2 induced metabolic perturbations in Physarum polycephalum with metabolomics

作　　者：张天任 闵武霞 夏沁怡 李佳琦 易思卓 张建华 刘士德 ZHANG Tianren;MIN Wuxia;XIA Qinyi;LI Jiaqi;YI Sizhuo;ZHANG Jianhua;LIU Shide(College of Life Sciences and Oceanography,Shenzhen University,Shenzhen Key Laboratory of Microbial Genetic Engineering,Shenzhcn 518060)

机构地区：[1]深圳大学生命与海洋科学学院深圳市微生物基因工程重点实验室,深圳518060

出　　处：《环境科学学报》2018年第9期3762-3769,共8页Acta Scientiae Circumstantiae

基　　金：深圳市科技研发资金基础研究计划(No.JCYJ20170302144210545;JCYJ20140418193546107;JC201105130450A);中国博士后科学基金(No.2017M622755);国家自然科学基金(No.31070043)~~

摘　　要：纳米TiO_2(n TiO_2)具有广泛的应用价值.但n TiO_2在阳光或紫外线下具有很强的氧化作用,可对细胞产生强烈的毒害作用,因此其对生态环境的可能破坏作用值得关注.有关n TiO_2在暗环境中对生物的毒性作用报道还不多,还不能全面揭示n TiO_2的毒性作用机理.代谢组学分析是发现代谢差异、识别被干扰的代谢途径、了解n TiO_2毒性作用机理、评估n TiO_2细胞毒性的重要手段.本文采用GC/MS(气相色谱/质谱)技术检测了n TiO_2在暗环境下对多头绒泡菌原质团(单细胞)代谢组的影响,根据多变量识别模式分析发现了60余个被n TiO_2显著干扰的代谢物.这些代谢物涉及糖代谢、氨基酸代谢、核苷酸代谢、多胺生物合成、次生代谢途径等,为全面了解n TiO_2对细胞的毒性作用机理提供了有益补充.Although widely utilized,Nano-sized TiO2（ n TiO2） has a strong oxidation effect under solar or UV irradiation,which is toxic to cells and its effect on the ecological environment is worthy of attention. There are few reports about the toxic effects of n TiO2 on organisms under the dark environment and the toxic mechanism of n TiO2 is not fully understood. Metabolomic analysis is an important to discover metabolic differences,identify disturbed metabolic pathways,understand the mechanism of toxicity of n TiO2 and assess cytotoxicity of n TiO2. In the present study,gas chromatography mass spectrometry（ GC/MS）-based metabolomics was used to investigate the effect of n TiO2 on cells（ Physarum polycephalum） under dark conditions.According to the multivariate pattern recognition analysis,more than 60 metabolites significantly interfered by n TiO2 were found. These metabolites involved in glycometabolism,amino acid metabolism,nucleotide metabolism,polyamine biosynthesis and secondary metabolic pathways,etc,which provided a useful supplement for a comprehensive understanding of the mechanism of toxicity of n TiO2 on cells.

关 键 词：纳米TiO2 多头绒泡菌 代谢组学 细胞毒性 

分 类 号：X171.5[环境科学与工程—环境科学]