抗瘤增效颗粒急性毒性实验及对Lewis肺癌小鼠抗肿瘤增效作用  

Acute toxicity test of anti-tumor synergistic granule and its anti-tumor synergistic effect on Lewis lung cancer mice

作　　者：赵晓珍[1] 吴中华[2] 张继全[3] 李更青 白月琴[1] 刘畅[1] ZHAO Xiaozhen;WU Zhonghua;ZHANG Jiquan(Department of Oncology,Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 20003)

机构地区：[1]上海中医药大学附属龙华医院肿瘤科,上海200032 [2]上海中医药大学分子生物与药理实验室,上海201203 [3]上海中医药大学中药现代制剂技术教育部工程研究中心,上海201203

出　　处：《河北中医》2018年第9期1384-1387,1393,共5页Hebei Journal of Traditional Chinese Medicine

基　　金：上海市进一步加快中医药事业发展三年行动计划(2014年-2016年)(编号:ZY3-JSFC-2-2006)

摘　　要：目的观察抗瘤增效颗粒的急性毒性实验及对Lewis肺癌小鼠的抗肿瘤增效作用。方法将40只ICR小鼠(雌雄各半)随机等分为4组,分别为阴性对照组、抗瘤增效颗粒低剂量组、抗瘤增效颗粒中剂量组和抗瘤增效颗粒高剂量组,每组各10只(雌雄各半),分别予0.9%氯化钠注射液0.4 mL/10 g及等容积抗瘤增效颗粒溶液(生药含量分别为312、468、624 g/kg)灌胃,每日1次,连续2周。每次灌胃后4 h内密切观察各组小鼠外观、体征、行为活动,然后每日上午、下午观察外观、体征、行为活动及粪便性状等,并分别于灌胃前及灌胃7、14 d称各小鼠体质量;2周后解剖各组小鼠,肉眼大体观察各小鼠脏器及组织变化。另取C57BL-6小鼠制备Lewis肺癌模型,选取造模成功的小鼠40只,随机等分为模型组、顺铂(DDP)治疗组、抗瘤增效颗粒低剂量+DDP组、抗瘤增效颗粒中剂量+DDP组和抗瘤增效颗粒高剂量+DDP组,模型组予0.9%氯化钠注射液0.4 mL/10 g灌胃;DDP治疗组、抗瘤增效颗粒低剂量+DDP组、抗瘤增效颗粒中剂量+DDP组和抗瘤增效颗粒高剂量+DDP组均于第1、3、5 d予DDP溶液1 mL(含DDP 0.1 mg)腹腔注射,同时分别予0.9%氯化钠注射液、抗瘤增效颗粒溶液(生药含量分别为0.6、0.8、1 g/mL)0.4 mL/10 g灌胃,每日1次,连续21 d。观察比较5组抑瘤率、肿瘤体积及组织形态学变化。结果急性毒性实验结果显示,4组小鼠外观、体征、行为活动及粪便性状均未发现异常,灌胃前及灌胃7、14 d体质量未见明显异常变化,各脏器组织均未发现异常。药效学实验结果显示,与模型组相比,DDP治疗组、抗瘤增效颗粒低剂量+DDP组、抗瘤增效颗粒中剂量+DDP组和抗瘤增效颗粒高剂量+DDP组小鼠瘤质量均显著减轻(P<0.05),肿瘤体积均缩小(P<0.05),且抗瘤增效颗粒中剂量+DDP组和抗瘤增效颗粒高剂量+DDP组小鼠瘤质量低于DDP治疗组(P<0.05),抑瘤率高于DDP治疗组(P<0.05),肿�Objective To observe the Acute toxicity test of anti-tumor synergistic granule and its anti-tumor synergistic effect on Lewis lung cancer mice. Methods 40 ICR mice（ male and female） were randomly divided into 4 groups,namely negative control group,anti-tumor synergistic granule low dose group,anti-tumor synergistic granule medium dose group and anti-tumor synergistic granule high dose group. Each group consisted of 10（ male and female）,respectively. The mice were given 0. 9% sodium chloride injection 0. 4 mL/10 g and the same volume of anti-tumor synergistic granule solution（ the crude drug content was 312,468,624 g/kg respectively）once a day for 2 weeks,and the appearance,physical signs and behavioral activities of the mice in each group were closely observed within 4 hours after each administration.The appearance,signs and behaviors of the mice in each group were closely observed within 4 hours after the second administration,and then the appearance,signs,behavioral activities and fecal traits were observed every morning and af-ternoon. The weight of mice was measured before administration and 7 and 14 days after administration,and the changes of organs and tissues were observed with naked eyes after 2 weeks. Another 40 C57 BL-6 mice were randomly divided into model group,DDP treatment group,anti-tumor synergistic granule low dose ＋ DDP group,anti-tumor synergistic granule medium dose ＋ DDP group and anti-tumor synergistic granule high dose ＋ DDP group.The model group was given 0. 9% sodium chloride injection 0. 4 mL/10 g daily. The DDP treatment group,anti-tumor synergistic granule low dose ＋ DDP group,midium dose ＋ DDP group and high dose ＋ DDP group were all intraperitoneally injected by DDP solution 1 mL（ containing DDP 0. 1 mg） on the first,third and fifth days. At the same time,0. 9% sodium chloride injection and anti-tumor synergistic granule（ containing 0. 6,0. 8,1 g/m） were was administered 0. 4 mL/10 g every day for 21 d. The tumor inhibition rate,tumor volume and tumor

关 键 词：癌 Lewis 抗肿瘤剂(中药) 动物实验 急性毒性试验 

分 类 号：R734.2[医药卫生—肿瘤] R285.5[医药卫生—临床医学]