长治移民与非移民血浆叶酸及代谢相关基因多态性比较  

Plasma folic acid and polymorphisms of folic acid metabolism related genes among migrants and non-migrants in Changzhi city

作　　者：崔国艳 杨建洲[2] 陈云霞 崔宇竹 CUI Guo-yan;YANG Jian-zhou;CHEN Yun-xia(Department of Microbiology,Branch of Basic Medicine,Changzhi Medical College,Changzhi,Shanxi Province 046000,China)

机构地区：[1]长治医学院基础医学部微生物教研室,山西046000 [2]长治医学院公共卫生与预防教研室

出　　处：《中国公共卫生》2018年第9期1270-1273,共4页Chinese Journal of Public Health

基　　金：山西省回国留学人员科研资助项目(2014-072);山西省自然科学基金(2016011092)

摘　　要：目的探讨山西省长治市移民与非移民人群血浆叶酸含量、亚甲基叶酸还原酶MTHFRC677T和甲硫氨酸合成酶MSA2756G位点多态性分布特点,为进一步研究其与疾病的相关性提供依据。方法于2014年5月—2015年6月采用酶联免疫吸附试验(ELISA)对80名长治林州移民和108名长治本地健康人群测定血浆中叶酸含量。同时采用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP)分析长治市人群MTHFR677位点和MS2756位点基因多态性。结果移民组与非移民组人群叶酸含量差异有统计学意义(P=0.006)。移民组内不同性别、年龄和是否饮酒的人群之间叶酸水平差异有统计学意义(P<0.05);非移民组组内不同血型的人群间差异有统计学意义(P<0.05)。2组组间比较结果表明:女性、>60岁、体质指数(BMI)<18.5 kg/m2、不吸烟和不饮酒的人群叶酸水平差异有统计学意义,MTHFR、MS的基因型和等位基因频率比较差异有统计学意义(P<0.05)。结论在不同特征的长治移民与非移民人群中血浆叶酸含量不同,2组人群MTHFR和MS基因多态性分布存在明显差异。Objective To investigate the content of plasma folic acid（FA） and the gene polymorphisms of methylenetetrahydrofolate reductase（MTHFR C677 T） and methionine synthase（MS A2756 G） among migrants and nonmigrants in Changzhi city of Shanxi province, and to provide evidences for further research. Methods We recruited 80 migrants and 108 non-migrants without abnormal findings in gastroscopy examination at a hospital in Changzhi city from May 2014 to June 2015. Plasma FA was detected with enzyme-linked immunosorbent assay（ELISA） and MTHFR and MS gene polymorphisms were analyzed with polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）among the participants. Results There was a significant difference in plasma FA level between the migrants and nonmigrants（P = 0.006）. Among the migrants, the plasma FA level differed significantly by gender, age, and whether drinking alcohol（P〈0.05 for all）; while, among the non-migrants, FA level differed significantly by blood type（P〈0.05）. There were significant differences in plasma FA levels between the migrants and non-migrants for the participants aged 60 years,with the body mass index（BMI） of 18.5 kg/m2, non-smokers, and non-alcohol drinkers（P〈0.05 for all）. The MTHFR and MS genotypes and allele frequencies were different significantly between the migrants and non-migrants（all P〈0.05）.Conclusion The plasma FA level is different between migrants and non-migrants and various subgroups of the two populations. The distribution of MTHFR and MS gene polymorphisms are also significantly different between the two population groups.

关 键 词：移民与非移民 叶酸 亚甲基四氢叶酸还原酶 甲硫氨酸合成酶 基因多态性 

分 类 号：R735[医药卫生—肿瘤]