干旱胁迫下磷脂酶Dδ和9-脂氧合酶对拟南芥茉莉酸合成及种子萌发的影响  

Effects of phospholipase Dδ and 9-lipoxygenase on jasmonic acid synthesis and seed germination of Arabidopsis thaliana under drought stress

作　　者：陈璐[1] 刘博 安炎黄 赵敏[1] 王玥萱 杨宁[1] CHEN Lu;LIU Bo;AN Yan-huang;ZHAO Min;WANG Yue-xuan;YANG Ning(College of Life Sciences,Northwest Normal University,Lanzhou 730070,China)

机构地区：[1]西北师范大学生命科学学院,兰州730070

出　　处：《生态学杂志》2018年第9期2627-2636,共10页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(31660116);西北师范大学创新团队项目资助

摘　　要：以拟南芥哥伦比亚野生型(WT)、磷脂酶Dδ(PLDδ)缺失型突变体pldδ和9-脂氧合酶(9-LOX)缺失型突变体lox1、lox5实生苗为材料,以0.3 mol·L-1甘露醇模拟干旱胁迫,分析PLDδ和9-LOX参与干旱胁迫下拟南芥茉莉酸(JA)生物合成和在种子萌发中作用。结果表明:干旱胁迫显著提高PLDδ和LOX1基因表达以及PLD和LOX酶活性;干旱胁迫下,pldδ突变体幼苗的LOX活性和JA含量显著低于WT,外源添加磷脂酸(PA)后LOX活性和JA含量显著上升,并高于WT;干旱胁迫显著抑制pldδ、lox1和lox5突变体的种子萌发,以对lox1的抑制效果最为明显;干旱胁迫下PLD活性上升与PLDδ基因表达上调有关,LOX活性上升与LOX1和LOX5基因表达上调有关,其中LOX1基因起主要作用;PLDδ/PA位于9-LOX上游参与9-LOX诱导的JA合成过程;PLDδ、LOX1和LOX5基因均参与干旱胁迫下拟南芥的种子萌发,LOX1在此过程中作用最为明显;PLDδ和9-LOX均参与PA和JA介导的种子萌发过程。Using Colombia wild Arabidopsis thaliana（ WT）,phospholipase Dδ（ PLDδ） deletion mutant（ pldδ） and 9-lipoxygenase（ 9-LOX） deletion mutants lox1 and lox5 seedlings as materials,we examined the effects of PLDδ and 9-LOX on biosynthesis of jasmonic acid（ JA） and seed germination of A. thaliana under drought stress（ 0.3 mol·L-1 mannitol）. The results showed that drought stress markedly increased the activities of PLD and LOX and the gene expressions of PLDδ and LOX1. Under drought stress,the LOX activity and JA content in pldδ seedlings were significantly lower than those in WT. Both LOX and JA significantly increased after phosphatidic acid（ PA） addition and were higher than those in WT. Drought stress significantly inhibited seed germination of pldδ,lox1 and lox5 mutants,with the strongest inhibitory effect on lox1. Under drought stress,the enhancement of PLD activity was related to PLDδ expression while the increasing activity of LOX was related to LOX1 and LOX5 expressions,with the dominant role from LOX1. PLDδ/PA was located at the upstream of 9-LOX and involved in the JA synthesis.All PLDδ,LOX1 and LOX5 participated in seed germination of A. thaliana under drought stress,with a dominant role of LOX1 in this process. Both PLDδ and 9-LOX were involved in PA and JA-mediated seed germination.

关 键 词：干旱胁迫 茉莉酸 磷脂酶D 脂氧合酶 萌发 

分 类 号：Q945.78[生物学—植物学]