稀土元素铈对斑马鱼肝脏的遗传毒性  

Genotoxicity of rare-earth element cerium on zebrafish( Danio rerio ) livers

作　　者：贺彦斌 台培东[1] 孙梨宗[1,2] 郭橙 刘志红 丛仕龙 HE Yan-bin;TAI Pei-dong;SUN Li-zong;GUO Cheng;LIU Zhi-hong;CONG Shi-long(Key Laboratory of Pollution Ecology and Environmental Engineering,Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China;University of Chinese Academy of Sci-ences,Beijing 100049,China;Liaoning Shihua University,Fushun 113001,Liaoning,China;Deakin University,Victoria 001138,Australia）.)

机构地区：[1]中国科学院污染生态与环境工程重点实验室、中国科学院沈阳应用生态研究所,沈阳110016 [2]中国科学院大学,北京100049 [3]辽宁石油化工大学,辽宁抚顺113001 [4]Deakin University,Victoria 001138,Australia

出　　处：《生态学杂志》2018年第9期2786-2793,共8页Chinese Journal of Ecology

基　　金：国家重点研发计划项目(2016YFD0800305); 国家自然科学基金项目(21677151、41673132、31600372); 辽宁省自然科学基金项目(201601329)资助

摘　　要：以稀土元素中丰度最大的铈(Ce)为代表污染物,以斑马鱼(Danio rerio)为受试生物,采用RAPD-PCR和MSAP-PCR技术研究Ce对斑马鱼基因组DNA损伤及DNA甲基化的影响。结果表明,不同浓度Ce^(3+)(0、5、10和20μmol·L^(-1))胁迫斑马鱼28天后,Ce^(3+)主要在斑马鱼肝脏中富集(BCF:5.49~9.33),其次为腮(BCF:3.58~4.49)和肌肉(BCF:0.13~0.25);RAPD-PCR分析显示,Ce^(3+)(>10μmol·L^(-1))胁迫能够诱导斑马鱼肝脏DNA的损伤;MSAP-PCR分析显示,Ce^(3+)胁迫引起的斑马鱼肝脏基因组DNA甲基化总变化率分别为8.93%(CK)、9.12%(5μmol·L^(-1))、15.56%(10μmol·L^(-1))和28.83%(20μmol·L^(-1)),其中低浓度胁迫下,斑马鱼肝脏基因组DNA去甲基化(D)型显著增加,高浓度下,甲基化(M)型显著增加,DNA甲基化多态性亦随胁迫浓度的增大而变大;测序分析结果显示,这些特异性甲基化条带与zinc finger protein STZ/ZAT10、ABC transporter 1、cell cycle control phosphatase superfamily protein等基因具有较高的同源性;与RAPD标记相比,DNA甲基化标记对Ce^(3+)的胁迫响应具有高敏感性。研究结果深化了对稀土元素生物学效应的认识。Cerium（ Ce） is the most abundant rare-earth element. Using RAPD-PCR（ random amplified polymorphic DNA-PCR） and MSAP-PCR（ methylation-sensitive amplified polymorphism-PCR）,the effects of Ce on genomic DNA damage and DNA methylation in zebrafish（ Danio rerio） were examined. The results showed that,after the fish was exposed to different concentrations of Ce^3＋（ 0,5,10 and 20 μmol·L^-1） for 28 days,Ce^3＋was mainly enriched in the liver（ bio-concentration factors BCF： 5.49-9.33）,followed by gill（ BCF： 3.58-4.49） and muscle（ BCF： 0.13-0.25）. The results of RAPD-PCR showed that Ce^3＋（ 10 μmol·L^-1） could induce DNA damage in zebrafish livers. The results of MSAP-PCR showed that the change rates of total genomic DNA methylation of zebrafish livers caused by Ce^3＋stress were 8. 93%（ CK）,9.12%（ 5 μmol·L^-1）,15.56%（ 10 μmol·L^-1）,and 28.83%（ 20 μmol·L^-1）,respectively.The demethylation（ D） of genomic DNA in zebrafish livers significantly increased under the low concentration of Ce^3＋,while the methylation（ M） significantly increased under the high concentration of Ce^3＋. Meanwhile,the methylation polymorphism increased with the increases of Ce^3＋concentration. Results from sequencing analysis showed that these specific bands had high homology with the genes of zinc finger protein STZ/ZAT10,ABC transporter 1,and cell cycle control phosphatase superfamily protein. Compared to RAPD markers,DNA methylation markers were highly sensitive to Ce^3＋stress. These results contribute to our understanding of the biological effects of rare earth elements.

关 键 词：铈 斑马鱼 DNA损伤 DNA甲基化 MSAP-PCR 

分 类 号：X171.5[环境科学与工程—环境科学]