颞叶癫痫模型大鼠海马区血管形成素-1、血管内皮生长因子的表达  

Expression of Angiopoietin-1 and Vascular Endothelial Growth Factor in Hippocampus of Rats with Temporal Lobe Epilepsy and Its Effect on Temporal Lobe Epilepsy

作　　者：张晶[1] 翟中文[1] ZHANG Jing.ZHAI Zhong-wen.(Department of Neurosurgery, The Second Affiliated Hospital of Inner Mongolia Medical University, Huhhot 010030, China)

机构地区：[1]内蒙古医科大学第二附属医院神经外科,呼和浩特010030

出　　处：《神经损伤与功能重建》2018年第9期444-447,共4页Neural Injury and Functional Reconstruction

基　　金：内蒙古自治区卫生科研基金项目(No.2015A037)

摘　　要：目的:探讨颞叶癫痫模型大鼠海马区血管形成素-1(Ang-1)、血管内皮生长因子(VEGF)的表达及其在颞叶癫痫中的作用。方法:选取雄性Wistar大鼠60只,腹腔注射氯化锂-匹罗卡品及生理盐水分别建立颞叶癫痫模型组及对照组;观察大鼠行为学改变,比较2组大鼠海马区Ang-1、VEGF的动态表达过程。结果:模型组均可见动物出现行为呆滞、活动减少、凝视、前肢搔痒样动作或平衡不能等异常表现,部分伴有外周胆碱能反应,但症状程度较轻,持续数十分钟后可自行缓解。对照组动物无抽搐表现。第1小时,模型组的Ang-1表达较对照组减少(P<0.05),至第3天降至最低水平,后逐渐升高(P<0.05),至18 d接近正常水平(P<0.05);VEGF表达第1天升高(P<0.05),至第3天升至最高水平(P<0.05),后逐渐降低,至18 d接近正常水平(P<0.05)。结论:抽搐频繁时,Ang-1表达持续降低,VEGF表达持续升高;抽搐减少时,Ang-1和VEGF表达逐渐恢复正常。Ang-1与VEGF表达呈直线负相关关系。Objective： To investigate the expression of angiopoietin-1 （Ang-1） and vascular endothelial growth factor （VEGF） in the hippocampus of rats with temporal lobe epilepsy and its effect on temporal lobe epilepsy. Methods： Sixty male Wistar rats were obtained. Intraperitoneal injections of LiCl-pilocarpine or normal saline were administered to generate the temporal lobe epilepsy model group and control group, respectively. Rat behavior change was observed, and dynamic changes in the expression of Ang-1 and VEGF in the hippocampus were analyzed. Results： Epilepsy model group animals displayed sluggish behavior, decreased activity, fixed gaze, scratching motion in forearms, or imbalance and other abnormal behaviors. A portion of these rats also showed peripheral cholinergic reactions, but these symptoms presented at a lesser degree and spontaneously resolved after several minutes. Control group animals displayed no seizures. At 1 hour, the model group showed decreased Ang-1 expression compared to the control group （P〈0.05）; at 3 days, expression reached its lowest level, then gradually increased （P〈0.05）; at 18 days, expression approached normal levels （P〈0.05）. At 1 day, VEGF expression was increased （P〈0.05）; at 3 days, expression reached its highest level （P〈0.05） then gradually decreased; at 18 days, expression level was close to normal （P〈0.05）. Conclusion： Ang-1 expression decreases with frequent tics and returns to normal levels with reduced tics. VEGF expression increases with frequent tics and returns to normal levels with reduced tics. The expression of Ang-1 and VEGF demonstrates a negative linear correlation.

关 键 词：癫痫 促血管生成素-1 血管内皮生长因子 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.02[医药卫生—临床医学]