尼美舒利对胆管癌细胞Th1/Th2细胞因子分泌的影响  

Effect of Nimesulide on Secreting Th1/Th2 Cell Cytokines of Cholangiocarcinoma Cell Line

作　　者：孔琦[1] 黄强[2] 陈斌[1] 王道明[1] 朱家胜[1] 夏亚斌[1] KONG Qi;HUANG Qiang;CHEN Bin;WANG Daoming;ZHU Jiasheng;XIA Yabin(Department of Gastrointestinal Surgery,Yijishan Hospital Affiliated to Wannan Medical College,Wuhu 241001,China;Department of General Surgery,Anhui Provincial Hospital,Hejbi 230001,China)

机构地区：[1]皖南医学院弋矶山医院胃肠外科,安徽芜湖241001 [2]安徽省立医院普外科,安徽合肥230001

出　　处：《肿瘤基础与临床》2018年第4期287-289,共3页journal of basic and clinical oncology

基　　金：安徽省教育厅自然科学基金资助项目(编号:KJ2008B083)

摘　　要：目的探讨尼美舒利对胆管癌细胞Th1、Th2细胞因子分泌的影响。方法应用酶联免疫吸附试验检测胆管癌细胞HCCC-9810中白介素-2(IL-2)、IL-4、IL-6和干扰素γ(IFN-γ)等Th1、Th2细胞因子的表达,流式细胞仪检测尼美舒利对Th1、Th2细胞因子分泌的影响。结果尼美舒利作用后,随其药物浓度升高,胆管癌细胞HCCC-9810中IL-2、IFN-γ水平升高,而IL-4、IL-6水平下降,Th1/Th2比值升高(P <0. 05)。结论胆管癌细胞呈Th2优势状态,尼美舒利可能通过逆转Th1/Th2偏移调节细胞免疫,从而发挥抗肿瘤作用。Objective To explore the effect of nimesulide on secreting Thl and Th2 cell cytokines of cholangiocarcinoma cell line in vitro. Methods In the cholangiocarcinoma HCCC-9810 cells, interleukin-2 （IL-2） , IL4, IL-6 and interferon-γ （IFN-γ） were detected by enzyme-linked immunosorbent adsorption. The changes of Thl and Th2 cytokines were analyzed by flow cytometry. Results After being cultured with different concentration of nimesulide, IL-2 and IFN-γ concentration were higher, IL4 and IL-6 concentration were lower, and Th1/Th2 ratio was higher in the cholangiocarcinoma HCCC-9810 cells （ P 〈 0.05）. Conclusion Cholangiocarcinoma cells showed Th2 dominance, nimesulide can convert the imbalance of Th1/Th2 cytokines, and regulate cellular immunity to prevent tumor growth.

关 键 词：胆管肿瘤 T淋巴细胞 细胞因子 

分 类 号：R73-36[医药卫生—肿瘤] R735.8[医药卫生—临床医学]