miR-124靶向调控Notch 1通路影响肝癌细胞的增殖和迁移  

MiR-124 inhibits hepatocellular carcinoma cell proliferation and migration by modulating the Notch 1 signal pathway

作　　者：朱剑宇[1] 侯萌 余正平[3] 庄磊[3] ZHu Jian-yu;HOu Meng;Yu Zheng-ping;ZHUANG Lei(Department of Trauma Surgery;Department of Radiation and Chemotherapy;Department of Hepatobiliary Surgery,the First Affliated Hospital of Wenzhou Medical University,Wenzhou,Zhejiang 325000,China)

机构地区：[1]温州医科大学附属第一医院创伤外科,浙江温州325000 [2]温州医科大学附属第一医院放化疗科,浙江温州325000 [3]温州医科大学附属第一医院肝胆外科,浙江温州325000

出　　处：《肝胆胰外科杂志》2018年第5期399-402,425,共5页Journal of Hepatopancreatobiliary Surgery

基　　金：温州市科技局项目(Y20160046)

摘　　要：目的探索微小RNA-124(mi R-124)通过Notch1信号通路对肝细胞性肝癌(HCC)细胞增殖和迁移的影响。方法将肝癌细胞Hep G2和人正常肝细胞HL7702作为受试对象。采用实时荧光定量PCR(q RT-PCR)法检测细胞中mi R-124的表达。Hep G2细胞转染mi R-124模拟物(mi R-124 mimic)后,CKK-8法检测细胞增殖活性,Transwell法检测细胞迁移能力。Western blotting检测Notch1信号通路表达。结果在Hep G2细胞中,mi R-124表达低于人正常肝细胞HL7702[(1.00±0.00)vs(21.32±1.02);P<0.05];与对照组(无处理的Hep G2细胞)相比,mi R-124 mimic转染组中Hep G2细胞增殖明显降低[(0.44±0.01)vs(0.21±0.01);P<0.05],细胞迁移能力也降低[(12.00%±2.00%)vs(5.67%±1.52%);P<0.05];mi R-124过表达可以明显抑制Hep G2细胞内Notch1信号通路蛋白表达[(100.00%±0.00%)vs(36.46%±2.36%);P<0.05]。结论本研究显示,mi R-124可靶向抑制Notch1表达,进而抑制HCC的增殖和迁移。Objective To investigate the influence of micro RNA-124（miR-124） on the proliferation and migration of heptocellular carcinoma（HCC）. Methods In this experiment, hepatocellular carcinoma cell line Hep G2 and human normal liver cell line HL7702 were subjects. The expression of miR-124 was examined by q RT-PCR. The miR-124 mimics was transfected into HCC cell line Hep G2 cells, and the cell proliferation and migration were examined by cell counting kit-8（CCK-8） assay and transwell assay, respectively. The expression of Notch1 signal pathway was determined by Western blotting. Results The expression level of miR-124 in Hep G2 was significantly lower than that in human normal liver cell line HL7702（P〈0.05）. For Hep G2 cells, after transfection, cellular proliferation and migration were inhibited by miR-124 mimic（P〈0.05）. Moreover, Notch1 signaling pathway was significantly inhibited by over-expression of miR-124 in Hep G2 cells. ConclusionOur study suggests that miR-124 may inhibit the heptocellular carcinoma cell proliferation and migration by modulating the Notch1 signal pathway.

关 键 词：癌 肝细胞 微小RNA-124 Notch1信号通路 增殖 迁移 

分 类 号：R735.7[医药卫生—肿瘤]