An extraction-assay system： Evaluation on flavonols in plant resistance to Pb and Cd by supercritical extraction-gas chromatography  

作　　者：Xu Zhang Huanhuan Yang Xinlei Wang Wen Song Zhaojie Cui 

机构地区：[1]School of Environmental Science and Engineering, Shandong University, Jinan 250100, China [2]School of Life Science, Shandong University, Jinan 250100, China

出　　处：《Frontiers of Environmental Science & Engineering》2018年第4期55-62,共8页环境科学与工程前沿（英文）

摘　　要：In this research, supercritical carbon dioxide extraction （SFE） showed better extraction etlect when compared with Solid- liquid extraction （SLE）, Soxhlet extraction （SEt and Ultrasonic extraction （UE）, not only in the rate but also the time. The comparison among these three extraction modifiers, including acetone, ethanol and methanol demonstrated that ethanol was preferred to SFE due to its high extraction effect and low toxicology. In addition, parameter of SFE, influence of temperature and pressure were investigated, and the best extraction effect was achieved at the optima conditions, temperature of 40℃ and the pressure of 35 MPa. Thus, SFE is a highly effective method for flavonols extraction, requiring minimum energy and producing non-toxic byproduct. SFE-GC system is applied for the evaluation on flavonols that plays a key role in plant resistance to heavy metal, with its content and synthetase gene expression significantly increasing in plant when threatened by heavy metal. Besides, results indicated that flavonols can improve plant resistance to oxidative stress by quenching the redundant ROS in matrix.In this research, supercritical carbon dioxide extraction （SFE） showed better extraction etlect when compared with Solid- liquid extraction （SLE）, Soxhlet extraction （SEt and Ultrasonic extraction （UE）, not only in the rate but also the time. The comparison among these three extraction modifiers, including acetone, ethanol and methanol demonstrated that ethanol was preferred to SFE due to its high extraction effect and low toxicology. In addition, parameter of SFE, influence of temperature and pressure were investigated, and the best extraction effect was achieved at the optima conditions, temperature of 40℃ and the pressure of 35 MPa. Thus, SFE is a highly effective method for flavonols extraction, requiring minimum energy and producing non-toxic byproduct. SFE-GC system is applied for the evaluation on flavonols that plays a key role in plant resistance to heavy metal, with its content and synthetase gene expression significantly increasing in plant when threatened by heavy metal. Besides, results indicated that flavonols can improve plant resistance to oxidative stress by quenching the redundant ROS in matrix.

关 键 词：FLAVONOLS Heavy metal Supercritical carbon dioxide extraction （SEE）GC Plant resistance 

分 类 号：TP391.41[自动化与计算机技术—计算机应用技术] TS255.1[自动化与计算机技术—计算机科学与技术]