Preparation and chromatographic evaluation of a chiral stationary phase based on carboxymethyl-β-cyclodextrin for high-performance liquid chromatography  

作　　者：Min Zhou Yuande Long Yonggang Zhi Xiaoying Xu 

机构地区：[1]Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 640041, China [2]University of Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Chinese Chemical Letters》2018年第9期1399-1403,共5页中国化学快报（英文版）

摘　　要：A chiral stationary phase （CSP） was prepared by chemically bonding carboxymethyl-β-cyclodextrin （CM- β-CD） onto 3-aminopropyl silica gel through amidation reaction in water solution and was characterized by Fourier transform infrared spectroscopy （FT-IR）, element analysis （EA） and thermal gravimetry analysis （TGA）. The chromatographic performance was evaluated with 24 racemates under reversedphase conditions, The effect of salt, organic modifier, mobile phase pH and structures of analytes were discussed. In comparison with native β-CD bonded column, CYCLOBOND 12000, CM-β-CD CSP exhibited enhanced enantioseparation.A chiral stationary phase （CSP） was prepared by chemically bonding carboxymethyl-β-cyclodextrin （CM- β-CD） onto 3-aminopropyl silica gel through amidation reaction in water solution and was characterized by Fourier transform infrared spectroscopy （FT-IR）, element analysis （EA） and thermal gravimetry analysis （TGA）. The chromatographic performance was evaluated with 24 racemates under reversedphase conditions, The effect of salt, organic modifier, mobile phase pH and structures of analytes were discussed. In comparison with native β-CD bonded column, CYCLOBOND 12000, CM-β-CD CSP exhibited enhanced enantioseparation.

关 键 词：ENANTIOSEPARATION Carboxymethyl-β-cyclodextrin Chiral stationary phase Chemical bonding REVERSED-PHASE 

分 类 号：O657.72[理学—分析化学]