新孢子虫NcGRA7与NcGRA2串联基因的真核载体构建与体外瞬时表达  

Construction of tandem gene eukaryotic vector and transient expression of Nospora canium NcGRA7 and NcGRA2

作　　者：金春梅[1] 胡楠[1] 蔡锦顺[1] 于龙政[1] JIN Chun-mei;HU Nan;CAI Jin-shun;YU Long-zheng(Department of Veterinary Medicine,College of Agriculture,Yanbian University,Yanji,Jilin 133002,China)

机构地区：[1]延边大学农学院动物医学系

出　　处：《中国兽医学报》2018年第9期1731-1734,共4页Chinese Journal of Veterinary Science

基　　金：吉林省教育厅基金资助项目(吉教科合字[2016]第258号,JJKH20170463KJ)

摘　　要：本研究为构建新孢子虫NcGRA7与NcGRA2串联基因的真核表达载体,以SOE-PCR技术得到新孢子虫NcGRA7-NcGRA2串联基因,并先克隆到pMD-19T载体上,经测序未发现移码后,再克隆到pDNA3.1载体上,用IFA进行体外瞬时表达的鉴定。结果表明,pDNA3.1-NcGRA7-NcGRA2串联基因的真核表达载体构建成功,在体外真核细胞内能表达外源蛋白。In this research,in order to construct the tandem gene eukaryotic vector,Nospora canium NcGRA7 and NcGRA2 gene were linked by using the SOE-PCR technique,and then inserted into pMD-19 Tvector.After sequencing,the tandem gene NcGRA7-NcGRA2 was cloned into pDNA3.1 vector.The transient expression of pDNA3.1-NcGRA7-NcGRA2 in vitro was identified by using IFA method.The results showed that pDNA3.1-NcGRA7-NcGRA2 vector had been constructed successfully,which could express foreign protein in vitro.

关 键 词：新孢子虫 串联基因 真核载体 

分 类 号：S852.723[农业科学—基础兽医学]