美味猕猴桃过氧化物酶基因AdPOD27的密码子偏好特征分析  

Analysis of Codon Bias Characteristic of a Peroxidase Gene AdPOD27 in Actinidia deliciosa

作　　者：赖瑞联[1] 冯新[1] 高敏霞[1] 钟春水 胡艺贤 吴如健[1] 陈义挺[1] LAI Ruilian;FENG Xin;GAO Minxia;ZHONG Chunshui;HU Yixian;WU Rujian;CHEN Yiting(Fruit Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China)

机构地区：[1]福建农业科学院果树研究所,福建福州350013

出　　处：《热带作物学报》2018年第7期1344-1350,共7页Chinese Journal of Tropical Crops

基　　金：福建省自然科学基金项目(No.2017J01044);福建省农业科学院博士科研启动基金项目(No.DC2017-2);福建省农业科学院项目(No.A2016-2);福建省农业科学院创新团队项目(No.STIT2017-3-6)

摘　　要：为了解美味猕猴桃过氧化物酶基因Ad POD27的密码子偏好特征,本研究采用Codon W1.4.2、SPSS19.0、MEGA5.2和EMBOSS等软件分析Ad POD27的密码子偏好性参数,同时对单子叶植物和双子叶植物间POD27的密码子使用规律进行比较。结果表明,Ad POD27有效密码子数(ENc)、密码子G和C含量(GC)、密码子第3位G或C的含量(GC3s)分别为50.65、0.443和0.429,意示着Ad POD27密码子偏好性较弱但偏好使用含有较多A和T并以A或T结尾的密码子;单子叶植物和双子叶植物平均ENc值分别为36.93和52.25,单子叶植物POD27密码子偏好性普遍强于双子叶植物;聚类分析结果表明,物种间具有严格的密码子偏好性规律。此外,Ad POD27与猕猴桃基因组密码子使用频率存在一定差异。本研究结果为进一步开展Ad POD27基因功能和分子进化研究提供了科学依据。To explore codon usage bias（CUB） characteristic of one peroxidase gene of Actinidia deliciosa（Ad POD27）, CUB parameters were analyzed by Codon W1.4.2, SPSS19.0, MEGA5.2 and EMBOSS in this study, and the CUB patterns between dicotyledon and monocotyledon were compared. The results showed that the effective number of codon（ENc）, GC content（GC） and GC content on the 3 rd site（GC3 s） was 50.65, 0.443 and 0.429, which indicated that CUB level of Ad POD27 was low, but it was bias toward the synonymous codons with A or T on the 3 rd site. The average value of ENc in dicotyledon and monocotyledon was 36.93 and 52.25 respectively, meaning the CUB level of POD27 in monocotyledon was higher than that in dicotyledon. Based on phylogeny tree analysis, there were strict codon usage rules in different plants. In addition, there were some differences of codon usage frequency between Ad POD27 and kiwifruit genome. This study would provide a scientific basis for further research of gene function and molecular evolution of Ad POD27.

关 键 词：美味猕猴桃 过氧化物酶 密码子偏好性 聚类分析 

分 类 号：S663.4[农业科学—果树学]