利用生物信息学方法鉴定和分析禾谷炭疽菌Rab蛋白家族  

Identification and Analysis of Rab Proteins in Colletotrichum graminicola by Bioinformatic Approach

作　　者：李源[1] 步伟洋 鲁国东[1] 齐尧尧 LI Yuan;BU Welyang;LU Guodong;QI Yaoyao(College of Agriculture and Forestry Science,Linyi University,Linyi,Shandong 276005,China;College of Plant Protec-tion,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学植物保护学院,福建福州350002 [2]临沂大学农林科学学院,山东临沂276005

出　　处：《热带作物学报》2018年第7期1416-1422,共7页Chinese Journal of Tropical Crops

基　　金：临沂大学博士启动基金(No.LYDX2016BS065)

摘　　要：Rab蛋白作为分子开关,调控着丝状真菌中囊泡运输的各个过程。为明确禾谷炭疽菌(Colletotrichum graminicola)中Rab蛋白家族成员的数量和理化特征,本研究利用生物信息学的方法对禾谷炭疽菌中的Rab蛋白进行比对搜索,并对得到的Rab蛋白进行理化性质和系统进化分析,以期望为进一步阐明其功能提供理论指导。结果表明,禾谷炭疽菌中共有10个Rab蛋白候选基因,都编码小分子的亲水蛋白,编码产物都定位于胞内具有膜结构的细胞器上,其中有一半为稳定蛋白。此外,该Rab蛋白家族的绝大多数成员在丝状真菌中的进化是保守的,都具有保守的结构域,但是该家族个别成员与同属的黄瓜炭疽菌(Colletotrichum orbiculare)中的同源蛋白的进化距离较远,暗示了禾谷炭疽菌为了适应环境和更好地侵染植物进化出了可能具有特异性功能的Rab蛋白。本研究的结果将为深入明确Rab蛋白家族的具体功能奠定基础,期望为进一步设计更加合理有效的玉米炭疽病防治策略提供新思路。Rab proteins regulate the intracellular vesicle trafficking events in filamentous fungi as molecular switches. In order to clarify the quantity and the physical and chemical characteristics of Rab family proteins in Colletotrichum graminicola, ten candidate Rab proteins were identified from the genome of C. graminicola by bioinformatic approach, the physical and chemical properties and phylogenetic relationship were analyzed, aimed to provide a theoretical guidance for further clarifying the functions. The results showed that all the proteins were small molecule hydrophilic proteins, located in the intracellular membrane-bound organelles and half of them were stable proteins. In addition, most of Rab proteins in C. graminicola were evolutionarily and structurally conserved among filamentous fungi, but individual members possessed relatively far phylogenetic relationship when compared with homologs in Colletotrichum orbiculare, suggesting that some Rab proteins in C. graminicola might have evolved specific functions in order to better adapt to the environment and infect plants. The study would lead to a better understanding of the exact functions of Rab proteins in C. graminicola, which woulod further provide new insights into design better strategies to control maize anthracnose.

关 键 词：禾谷炭疽菌 RAB蛋白 进化分析 保守结构域 

分 类 号：S432.1[农业科学—植物病理学]