结肠癌病灶内Runt相关转录因子2与增殖基因、抑癌基因、血管新生分子的相关性研究  

Correlation research of Runt-related transcription factor 2 with proliferation genes,tumor suppressor genes and angiogenesis molecules in colon cancer lesions

作　　者：向春华[1] 鲍峰[1] 冯俊[1] XIANG Chun-hua;BAO Feng;FENG Jun(General Surgery Department,Mianyang Central Hospital of Sichuan Province,Mianyang 621000,China)

机构地区：[1]四川省绵阳市中心医院普外科,四川绵阳621000

出　　处：《海南医学院学报》2018年第18期1643-1646,共4页Journal of Hainan Medical University

基　　金：四川省卫计委普及应用项目(16PJ191)~~

摘　　要：目的:探讨结肠癌病灶内Runt相关转录因子2(RunX2)与增殖基因、抑癌基因、血管新生分子的相关性。方法:90例原发性结肠癌患者作为结肠癌组、68例良性结肠息肉患者作为结肠息肉组,对比两组病灶组织中RunX2及增殖基因、抑癌基因、血管新生分子表达量的差异,进一步采用Pearson检验评估结肠癌组织中RunX2表达量与增殖基因、抑癌基因、血管新生分子表达量的相关关系。结果:结肠癌组病灶中RunX2mRNA的表达量高于结肠息肉组。结肠癌组病灶中增殖基因GTPBP4、HOXB7、ZNF331、ADAM17、HSP60 mRNA的表达量高于结肠息肉组;抑癌基因ATF3、FOXN3、OTUD1、NDRG2mRNA的表达量低于结肠息肉组;血管新生分子Musashi 1、NF-κB、RegⅣ、STAT3mRNA的表达量高于结肠息肉组。结肠癌病灶中RunX2mRNA表达量与上述增殖基因、抑癌基因、血管新生分子表达量直接相关。结论:结肠癌病灶内RunX2异常高表达,且具体表达量与癌细胞的增殖活性、血管新生活性均呈正相关,是导致结肠癌发生发展的重要分子靶点。Objective： To investigate the correlation of Runt-related transcription factor 2 （RunX2） with proliferation genes, tumor suppressor genes and angiogenesis molecules in colon cancer lesions. Methods： 90 patients with primary colon cancer were enrolled in colon cancer group, 68 patients with benign colon polyps were enrolled in colon polyps group, the differences in the expression levels of RunX2, proliferation genes, tumor suppressor genes and angiogenesis molecules in the two groups of lesions were compared, and Pearson test was further used to evaluate the correlation of RunX2 expression level with proliferation gene, tumor suppressor gene and angiogenesis molecule expression levels in colon cancer tissues. Results： RunX2 mRNA expression level in the lesions of colon cancer group was higher than that of colon polyps group（ P 〈0.05）. Proliferation genes GTPBP4, HOXB7, ZNF331, ADAM17 and HSP60 mRNA expression levels in the lesions of colon cancer group were higher than those of colon polyps group（ P 〈0.05）; tumor suppressor genes ATF3, FOXN3, OTUD1 and NDRG2 mRNA expression levels were lower than those of colon polyps groups（ P 〈0.05）; angiogenesis molecules Musashi 1, NF-κB, RegⅣ and STAT3 mRNA expression levels were higher than those of colon polyps group（ P 〈0.05）. RunX2 mRNA expression level in the colon cancer lesions was directly correlated with the expression levels of the above proliferation genes, tumor suppressor genes and angiogenesis molecules. Conclusion： RunX2 expression is abnormally high in colon cancer lesions, the specific expression level is positively correlated with cancer cell proliferation activity and angiogenesis activity, and it is an important molecular target that can lead to the occurrence and development of colon cancer.

关 键 词：结肠癌 Runt相关转录因子2 增殖基因 抑癌基因 血管新生分子 

分 类 号：R735.35[医药卫生—肿瘤]