miR-370在原发性中枢神经系统淋巴瘤的表达和预后意义  

miR-370 expression in primary central nervous system lymphoma and its relationship with prognosis

作　　者：吕骏晖 王坤[1] 万英锋 王义荣[1] 宋正飞[1] 牛焕江[1] 李新伟[1] LYU Junhui,WANG Kun,WAN Yingfeng,et al.(Department of Neurosurgery, Sir Run Run Shaw Hospital, Medical School of Zhejiang University, Hangzhou 310016, Chin)

机构地区：[1]浙江大学医学院附属邵逸夫医院神经外科,浙江杭州310016

出　　处：《全科医学临床与教育》2018年第5期498-500,共3页Clinical Education of General Practice

基　　金：浙江省自然科学基金(LY14H160025);国家自然科学基金(81402044);浙江省自然科学基金(LY14H160017)

摘　　要：目的评估miR-370在原发性中枢神经系统淋巴瘤(PCNSL)的表达和预后意义。方法通过即时聚合酶链锁反应(qRT—PCR)测定62例PCNSL的miR-370的表达水平,并以12例患者的颈部淋巴结组织进行miR-370测定作为对照。并对62例PCNSL患者的临床特征、手术以及治疗方案、生存时间等进行收集和随访。结果 miR-370在PCNSL组织中相对表达比较水平明显低于淋巴结组织,差异有统计学意义(t=5.11,P<0.05)。单一脑叶患者的miR-370表达明显多于多个脑叶患者,差异有统计学意义(t=7.13,P<0.05)。不同性别、年龄、不同的手术方式、治疗方案组间miR-370表达差异无统计学意义(t分别=2.77、1.81、3.05、2.43,P均>0.05)。年龄、肿瘤位置、手术方案、miR-370的表达水平是影响PCNSL预后的影响因素(HR分别=0.11、1.95、0.37、0.01,P均<0.05)。结论 miR-370在PCNSL细胞中异常低表达,是一个重要的预后预测因子。此外,年龄、肿瘤位置和手术方案也是影响PCNSL患者预后的重要因素。Objective To evaluate the expression and prognostic significance of miR-370 in primary central nervous system lymphoma（PCNSL）. Methods Immediate polymerase chain reaction（qRT-PCR）was used to detect the expressions of miR-370 in 62 cases of PCNSL,and miR-370 was detected in 12 cases of cervical lymph node tissues as control. The clinical features,surgical approach,treatment and median survival time of the patients were followed-up.Results The relative expression level of miR-370 in PCNSL tissues was significantly lower than that in lymph node tissues（t=5.11,P〈0.05）. The expression of miR-370 in patients with single lobe was significantly higher than that in patients with multiple lobes（t=7.13,P〈0.05）. There was no significant difference in the expression of miR-370 between the groups of different sex,age,surgery method and treatment plan（t=2.77,1.81,3.05,2.43,P〉0.05）. Age,tumor location,surgical procedure,and the expression of miR-370 were the influencing factors for the prognosis of PCNSL（HR=0.11,1.95,0.37,0.01,P〈0.05）. Conclusion The miR-370 is an important prognostic factor with the low expression in PCNSL. In addition,age,tumor location and surgery method are also important factors that were related to the prognosis of PCNSL.

关 键 词：miR-370 实时定量聚合酶链反应 原发性中枢神经系统淋巴瘤 预测因子 预后 

分 类 号：R739.4[医药卫生—肿瘤]