吡哆胺对AGEs干预的人RPE细胞的保护作用  

Protective effect of pyridoxamine on RPE cells treated with AGEs

作　　者：周敏[1] 黄焱[2] 赖巧玲 邓云平 Min Zhou1, Yan Huang2, Qiao -Ling Lai2, Yun -Ping(1 .Department of Ophthalmology, The Seventh Affiliated Hospital ofSun Yat-sen University, Shenzhen 518000, Guangdong Province,China; 2. Department of Ophthalmology and Optometry, FujianMedical University, Fuzhou 350004, Fujian Province, Chin)

机构地区：[1]中山大学附属第七医院眼科,中国广东省深圳市518000 [2]福建医科大学医学技术与工程学院眼视光系,中国福建省福州市350004

出　　处：《国际眼科杂志》2018年第10期1778-1781,共4页International Eye Science

基　　金：2016年福建省自然科学基金项目(No.2016J01155)~~

摘　　要：目的:观察吡哆胺(PM)对高级糖基化终末产物(AGEs)干预的RPE细胞的影响,探讨PM对RPE细胞的保护作用。方法:取传代至第3代的细胞进行分组:(1)对照组:含100mg/L BSA的DMEM培养基培养48h;(2) AGEs干预组:含200mg/L AGEs的DMEM培养基培养48h;(3) PM组:PM1组:含16mg/L PM+200mg/L AGEs的DMEM培养基培养48h; PM2组:含32mg/L PM+200mg/L AGEs的DMEM培养基培养48h。采用免疫组织化学法检测RAGE蛋白的表达;荧光染色法观察细胞内ROS的生成; Tunel法检测细胞凋亡情况。结果:AGEs可明显刺激RPE细胞内RAGE蛋白的表达和ROS的生成,增加细胞凋亡情况,而PM可抑制该过程,且具有一定的浓度依赖性。结论:PM能够抑制AGEs干预的RPE细胞内RAGE蛋白的表达和ROS生成,减少细胞凋亡。AIM： To observe the effects of pyridoxamine（ PM） on RAGE, ROS and apoptosis in RPE cells treated with advanced glycation end products（ AGEs）, and to investigate the protective effect of PM on RPE cells in diabetic retinopathy.METHODS： Primary cultured human RPE cells,the third generation of cells were synchronized with serum-free Dulbecco-modified Eagle medium for 24 h, and then grouped： 1） Control group： cultured with 100 mg/L BSA for 48 h; 2） AGEs-treated group： cultured with 200 mg/L AGEs for 48 h; 3） PM group： PM1 group： cultured with16 mg/L PM＋200 mg/L AGEs for 48 h; PM2 group： cultured with 32 mg/L PM ＋200 mg/L AGEs for 48 h. The expression of RAGE protein was detected by immunohistochemistry.The formation of ROS was observed by fluorescence microscopy. The apoptosis of cells was detected by TUNEL.RESULTS： The expression of RAGE protein,ROS and apoptosis of RPE cells in PM group were significantly lower than those in AGEs-treated group,and decreased with the increase of PM concentration.CONCLUSION： Pyridoxamine can inhibit the expressionof RAGE and the production of ROS,reduce apoptosis,and have a protective effect on RPE cells.

关 键 词：吡哆胺 高级糖基化终末产物 RAGE 视网膜色素上皮细胞 细胞凋亡 

分 类 号：R774[医药卫生—眼科]