电击法建立SD大鼠焦虑型心理应激-失眠模型的研究  

Study on establishment of anxiety type psychological stress-insomnia model in SD rats by electric shock

作　　者：张晓婷[1] 刘文超[1] 刘俊昌[1,2] 李明星 郭蕾 卡热买提.阿布都克然木 张赫 张新均 ZHANG Xiaoting;LIU Wenchao;LIU Junchang;LI Mingxing;GUO Lei;Karemaiti·Abudukeranmu;ZHANG He;ZHANG Xinjun(Xinjiang Medical University,Urumqi 830000,Xinjiang,China;Xinjiang Uygur Autonomous Region Hospital of Traditional Chinese Medicine,Urumqi 830000,Xinjiang,China)

机构地区：[1]新疆医科大学,新疆乌鲁木齐830000 [2]新疆维吾尔自治区中医医院,新疆乌鲁木齐830000

出　　处：《现代中西医结合杂志》2018年第30期3316-3319,共4页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：新疆维吾尔自治区中医民族医药管理局科技人才专项课题(2016-03-17)

摘　　要：目的建立SD大鼠焦虑型心理应激-失眠模型并进行评价,探讨其在科研领域的应用价值。方法将26只雄性SD大鼠随机分为2组,模型组14只采用不可逃避性足底电击方法建立焦虑型心理应激-失眠模型(选用25 V电压,通电与间歇1∶5的电击量,每天不定时剥夺大鼠睡眠时间2 h,共21 d),空白组12只正常喂养。21 d后观察2组大鼠体征、自主活动及学习记忆情况。结果模型组大鼠全身皮毛疏松散乱、发黄,眼色暗红,易激惹,且实验1,2,3周大鼠体质量均明显低于同期空白组(P均<0. 05);自主活动测试中,模型组的垂直得分和水平得分均明显高于空白组(P均<0. 05),修饰次数明显多于空白组(P <0. 05);跳台测试中,模型组大鼠潜伏期明显长于空白组(P <0. 05),错误次数明显多于空白组(P <0. 05)。结论电击法建立SD大鼠焦虑型心理应激-失眠模型方法简便、可靠、高效,可同时对多只大鼠进行模型建立,该造模方法可应用于探究焦虑型失眠的病因病机研究及焦虑型失眠疗效的评价。Objective It is to establish an anxiety type psychological stress-insomnia model in SD rats and evaluate it, and explore its application value in scientific research field. Methods Twenty-six male SD rats were randomly divided into 2 groups. Forteen cases in the model group was established anxiety type psychological stress-insomnia model by avoidable sole electroshock method （25 V voltage, electric shock volume of power up/ intermittence with 1/5, daily sleep deprivation time was 2 hours for 21 days）, twelve cases in the blank group were fed normally. After 21 days, the differences in signs, autonomous activities, learning and memory in the two group were observed.Results Rats in the model group was irritable, and with fur of loose, scattered and yellowed, and their eyes was dark red; the body weight of rats in experiment in 1, 2 and 3 weeks was significantly lower than that in the blank group at the same time （all P 〈0.05）. In the independent activity test, the vertical and horizontal scores of the model group were sig nificantly higher than those of the blank group （all P 〈 0.05）, the number of modification times was significantly higher than that of blank group （ P 〈0.05）. In the platform test, the incubation period of the model group was significantly longer than that of the blank group （ P 〈0.05）, the number of errors was significantly higher than that on the blank group （ P 〈0.05）.Conclusion The electroshock method is a simple, reliable and effective method to establish the anxiety type psychological stress-insomnia model of SD rats, and the models of anxiety and insomnia can be established in many rats at the same time. This method can be applied to explore the etiology and pathogenesis of anxiety insomnia, and also to evaluate the curative effect of anxiety insomnia.

关 键 词：动物模型 SD大鼠 焦虑 心理应激 失眠 

分 类 号：R-332[医药卫生]