慢性淋巴细胞白血病患者骨髓INF-γ、IL-2、IL-4、IL-10及IL-1β水平的变化及意义  

Changes and Significance of IFN-γ,IL-2,IL-4,IL-10 and IL-1β in Marrow of Patients with Chronic Lymphocytic Leukemia

作　　者：石沁 欧阳红梅[2] 宋建新[2] SHI Qin;OUYANG Hong-mei;SONG Jian-xin(Department of Clinical Laboratory,Yunxian People＇s HospitaI,Yunxian 675800,Yunnan,China;Hematology Diagnosis Room,the First People＇s Hospital of Yunnan Province,Kunming 650032,Yunnan,China)

机构地区：[1]云南省临沧市云县人民医院检验科,云南云县675800 [2]云南省第一人民医院血液学诊断室,云南昆明650032

出　　处：《医学信息》2018年第17期149-151,共3页Journal of Medical Information

摘　　要：目的探讨CLL患者骨髓中INF-γ、IL-2、IL-4、IL-10及IL-1β水平的变化及意义。方法选取我院初诊CLL患者66例为病例组,根据Binet分期分为BinetA期29例,BinetB期21例,BinetC期16例;选取同期来我院健康体检者30例为对照组,另取38例治疗中的CLL患者为治疗组。应用流式细胞仪分析各组患者骨髓INF-γ、IL-2、IL-4、IL-10、IL-1β表达,观察在CLL患者不同时期的表达差异。结果 IFN-γ、IL-2表达在CLL各组中均低于对照组(P<0.05),而BinetC期患者表达低于BinetB期(P<0.05),BinetB期低于BinetA期(P<0.05),与Binet分期呈负相关。INF-γ、IL-2在CLL治疗后虽高于BinetA期,但P>0.05,而低于对照组(P<0.05)。IL-4、IL-10、IL-1β表达高于对照组(P<0.05),BinetB期高于BinetA期(P<0.05),BinetC期高于BinetB期(P<0.05),与Binet分期呈正相关。治疗后IL-4、IL-10、IL-Iβ与BinetA期比较有所降低,但P>0.05,与对照组比较仍高于对照组(P<0.05)。结论 CLL患者骨髓微环境细胞因子表达紊乱,Th1类细胞因子INF-γ、IL-2随着CLL Binet分期的进展逐渐减低,IL-4、IL-10、IL-1β则逐渐增高,与CLL的发生、发展相关。Objective To investigate the changes and significance of INF-γ,IL-2,IL-4,IL-10 and IL-1β in marrow of CLL patients.Methods 66 patients with newly diagnosed CLL in our hospital were selected as the case group.According to Binet stage,there were 29 cases of BinetA stage,21 cases of BinetB stage and 16 cases of BinetC stage.30 cases of healthy physical examination in our hospital were selected as control group.The CLL patients in the treatment group were the treatment group.Another 38 patients with CLL under treatment were treated.The expressions of INF-γ,IL-2,IL-4,IL-10 and IL-1β in marrow of each group were analyzed by flow cytometry,and the expression differences in different periods of CLL patients were observed.Results The expressions of IFN-γ and IL-2 in CLL group were lower than those in control group（P〈0.05）,while those in BinetC stage were lower than BinetB stage（P〈0.05）,and BinetB stage was lower than BinetA stage（P〈0.05）,negatively correlated with Binet staging.INF-γ and IL-2 were higher than the BinetA phase after CLL treatment,but P〉0.05,which was lower than the control group（P〈0.05）.The expressions of IL-4, IL-10 and IL-1β were higher than those of the control group（P〈0.05）,the BinetB phase was higher than the BinetA phase（P〈0.05）, the BinetC phase was higher than the BinetB phase（P〈0.05）,positively correlated with Binet staging.After treatment,IL-4,IL-10,IL-Iβ and BinetA phase were decreased,but P〉0.05,which was still higher than the control group（P〈0.05）.Conclusion The expression of cytokines in bone marrow microenvironment of patients with CLL is disordered.The cytokines of Th1 type cytokines INF-γ and IL-2 decrease with the progression of CLL Binet stage,and IL-4,IL-10 and IL-1β gradually increase,and CLL occurrence and development are related.

关 键 词：慢性淋巴细胞白血病 骨髓 细胞因子 

分 类 号：R733.72[医药卫生—肿瘤]