口腔鳞癌病灶内HIF-1α变化与淋巴结转移及恶性生物学分子表达的相关性  

The correlation of the change of HIF-1α in oral squamous cell carcinoma lesion with lymph node metastasis and malignant biological molecule expression

作　　者：赵文[1] 叶蕾[1] 李慧琴[1] 贺娟[1] ZHAO Wen;YE Lei;LI Hui-qin;HE Juan(Department of Stomatology,the First Hospital of Yulin City in Shaanxi Province,Yulin,Shaanxi 718000,China)

机构地区：[1]陕西省榆林市第一医院口腔科,陕西榆林718000

出　　处：《海南医学院学报》2018年第17期1611-1614,共4页Journal of Hainan Medical University

基　　金：陕西省科学技术研究发展计划项目2011K13-02-06)~~

摘　　要：目的:研究口腔鳞癌病灶内缺氧诱导因子-1α(HIF-1α)变化与淋巴结转移及恶性生物学分子表达的相关性。方法:选择2012年3月～2017年12月期间在榆林市第一医院接受手术切除的口腔鳞癌患者作为研究对象,手术切除后留取口腔鳞癌病灶及癌旁病灶适量,测定HIF-1α、增殖基因、侵袭基因的mRNA表达量以及血管新生分子的含量。结果:口腔鳞癌病灶内HIF-1α、PCNA、CDK4、CDK6、Slug、β-catenin、MMP9的mRNA表达量以及VEGF、Ang-2、bFGF、COX-2的含量显著高于癌旁病灶,PDCD5、Smac、E-cadherin、RECK的mRNA表达量显著低于癌旁病灶;Pearson相关性分析显示,口腔鳞癌病灶内HIF-1α的mRNA表达量与PCNA、CDK4、CDK6、Slug、β-catenin、MMP9的mRNA表达量以及VEGF、Ang-2、bFGF、COX-2的含量呈正相关,与PDCD5、Smac、E-cadherin、RECK的mRNA表达量呈负相关。结论:口腔鳞癌病灶内高表达的HIF-1α能够促进癌细胞的增殖、侵袭以及病灶内的血管新生。Objective：To study the correlation of the change of hypoxia-inducible factor-1α（HIF-1α）in oral squamous cell carcinoma lesion with lymph node metastasis and malignant biological molecule expression.Methods：The patients with oral squamous cell carcinoma who underwent surgical resection in the First Hospital of Yulin between March 2012 and December 2017 were selected as the research subjects,and right amount of oral squamous cell carcinoma lesion and lesion adjacent to carcinoma were collected after surgical resection to determine the mRNA expression of HIF-1α,proliferation genes and invasion genes as well as the contents of angiogenesis molecules.Results：HIF-1α,PCNA,CDK4,CDK6,Slug,β-catenin and MMP9 mRNA expression as well as VEGF,Ang-2,bFGF and COX-2 contents in oral squamous cell carcinoma lesions were significantly higher than those in adjacent lesions whereas PDCD5,Smac,E-cadherin and RECK mRNA expression were significantly lower than those in adjacent lesions;Pearson correlation analysis showed that HIF-1α mRNA expression in oral squamous cell carcinoma lesions was positively correlated with PCNA,CDK4,CDK6,Slug,β-catenin and MMP9 mRNA expression as well as VEGF,Ang-2,bFGF and COX-2 contents,and negatively correlated with PDCD5,Smac,E-cadherin and RECK mRNA expression.Conclusion：Highly expressed HIF-1αin oral squamous cell carcinoma can promote the proliferation and invasion of cancer cells as well as the angiogenesis in the lesion.

关 键 词：口腔鳞癌 缺氧诱导因子-1Α 血管新生 增殖 侵袭 

分 类 号：R739.8[医药卫生—肿瘤]