甲状腺乳头状微小癌中微淋巴管密度和微血管密度的检测及意义  

Detection and Significance of MLD and MVD in Human Papillary Thyroid Micro-carcinoma

作　　者：于德浩 胡斌[2] 徐晋洋 李珀[2] 何常[2] YU Dehao;HU Bin;XV Jinyang;LI Po;HE Chang(Department of Pathology,Guizhou Medical University,Guiyang 550004,Guizhou,China)

机构地区：[1]贵州医科大学 [2]贵州医科大学病理学教研室,贵州贵阳550004

出　　处：《贵州医科大学学报》2018年第9期1033-1037,共5页Journal of Guizhou Medical University

摘　　要：目的:探讨甲状腺乳头状微小癌中微淋巴管密度(MLD)和微血管密度(MVD)的检测及意义。方法:收集60例甲状腺乳头状微小癌标本及20例结节性甲状腺肿组织标本,采用免疫组织化学Envision两步法检测两种标本中D2-40和CD31表达情况,分别定量计数D2-40阳性表达的MLD和CD31阳性表达的MVD,分析它们与甲状腺乳头状微小癌转移的关系,并分析甲状腺乳头状微小癌中MLD与MVD的相关性。结果:甲状腺乳头状微小癌中MLD与MVD值高于结节性甲状腺肿,差异有统计学意义(P <0. 05);有淋巴结转移的甲状腺乳头状微小癌标本MLD与MVD值明显高于无转移淋巴结标本,差异有统计学意义(P <0. 05);在甲状腺乳头状微小癌中,MLD与MVD呈正相关关系(r=0. 742,P <0. 05)。结论:检测D2-40和CD31标记的MLD和MVD,对预测肿瘤淋巴结转移有潜在应用价值。Objective：To investigate the detection and significance of MLD and MVD in papillary thyroid micro-carcinoma. Methods： 60 specimens of papillary thyroid micro-carcinoma and 20 specimens of nodular goiter tissues were collected. The expression of D2-40 and CD31 in the two kinds of specimens was detected by immunohistochemistry. The micro-lymphatic vessel density （MLD） and micro-vascular density （MVD） ,marked by D2-40 and CD31,in papillary thyroid micro-carcinoma and nodular goiter tissues were quantitatively counted.Also their correlation with lymph node metastasis in papillary thyroid micro-carcinoma were analyzed. Results： The values of MLD and MVD in papillary thyroid micro-carcinoma were higher than those in nodular goiter tissues, and the difference was statistically significant （ P〈 0.05）.The values of MLD and MVD in thyroid papillary microcarcinoma with lymph node metastasis were significantly higher than those without lymph node metastasis, and the difference was statistically significant（ P 〈0.05）. There was a positive correlation between MLD and MVD in papillary thyroid carcinoma （ r=0.742,P 〈0. 05）. Conclusion： The detection of MLD and MVD marked by D2-40 and CD31 has potential application in predicting lymph node metastasis.

关 键 词：微淋巴管密度 微血管密度 甲状腺肿瘤 甲状腺乳头状微小癌 免疫组织化学 

分 类 号：R736.1[医药卫生—肿瘤]