高危型HPV-DNA检测负荷量与宫颈病变程度的相关性分析  

Analysis of the correlation betweenthe load of high risk human papillomavirus DNA detection and the severity of cervical lesions

作　　者：霍墨杰 霍艳芬 苏俊泽 HUO Mojie;HUO Yanfen;SU Junze(Department of Obstetrics and Gynecology,Hengshui Second Hospital,Hengshui 053000,Hebei,China)

机构地区：[1]河北省衡水市第二人民医院妇产科,河北衡水053000

出　　处：《中国性科学》2018年第8期52-56,共5页Chinese Journal of Human Sexuality

基　　金：衡水市科技局抗HPV生物蛋白辅料治疗HPV高危感染合并宫颈CIN1的临床研究(15044)

摘　　要：目的:分析宫颈病变严重程度与高危型人乳头瘤病毒(HR-HPV) DNA检测负荷量的相关性。方法:选取河北省衡水市第二人民医院2016年11月至2018年01月收治的宫颈病变患者320例进行研究,以组织病理学诊断为金标准,根据宫颈病变严重程度分为宫颈组织正常或炎症(正常组) 52例,宫颈癌(宫颈癌组) 93例,宫颈上皮内瘤变(CIN组) 175例,三组合计320例。其中又将CIN组分为宫颈上皮内瘤变Ⅰ级(CINⅠ组) 58例、宫颈上皮内瘤变Ⅱ级59(CINⅡ组)例、宫颈上皮内瘤变Ⅲ级(CINⅢ组) 58例。所有患者的高危型HPV-DNA负荷量采用第二代杂交捕获技术进行检测。观察并比较各组患者的高危型HPV-DNA的检出情况及其负荷量检测情况,并采用Spearman相关性分析宫颈病变严重程度与高危型HPVDNA检测负荷量的相关性。结果:正常组、CIN组的高危型HPV-DNA检出率(30. 77%)、(66. 29%)显著低于宫颈癌组的高危型HPV-DNA检出率(96. 77%);正常组的高危型HPV-DNA检出率(30. 77%)显著低于CIN组的高危型HPV-DNA检出率(66. 29%); CINⅠ组、CINⅡ组的高危型HPV-DNA检出率(44. 83%)、(55. 93%)显著低于CINⅢ组的高危型HPV-DNA检出率(94. 83%); CINⅠ组的高危型HPV-DNA检出率(44. 83%)显著低于CINⅡ组的高危型HPV-DNA检出率(55. 93%),差异具有统计学意义(P <0. 05)。各组之间的高危型HPV-DNA负荷量相比,发现其高危型HPV-DNA负荷量并不完全随宫颈病变严重程度加重而逐渐增加,差异无统计学意义(P> 0. 05)。采用Spearman相关性分析对高危型HPV-DNA负荷量进行统计学处理,发现宫颈病变严重程度与高危型HPV-DNA负荷量不完全相关(P> 0. 05),差异无统计学意义。结论:患者的宫颈病变程度越严重,其高危型HPV-DNA的检出率也随之增加,但高危型HPV-DNA负荷量并不完全随宫颈病变严重程度加重而逐渐增加,即与宫颈病变的严重程度不完全相关。Objective：To analyze the correlation between the severity of cervical lesions and the load of high-risk human papillomavirus DNA （HPV-DNA）detection. Methods ：320 patients with cervical lesions in Heng shui Second Hospitalfrom November 2016 to January 2018 were included in the study. With the pathological diagnosis as the gold standard, the 320 patients were divided into normal or inflammatory cervical tissues group （normal group, n = 52）, cervical cancergroup （n = 93） and cervical intraepithelial neoplasia （CIN group, n = 175） according to the severity of cervical lesions. The 175 patients in the CIN group was further divided into cervical intraepi- thelial neoplasia grade I （ CIN Ⅰ group, n = 58）, cervical intraepithelial neoplasia grade Ⅱ （ CIN Ⅱ group, n = 59） and cervical intraepithelial neoplasia grade Ⅲ（ CIN Ⅲ group, n = 58 ）. The load of high-risk HPV-DNA was detected by second generation cross capture techniques. The detection results were observed and compared between groups and the correlation between the severity of cervical lesions and the detection load of high-risk HPV-DNA was analyzed by Spearman correlation. Results ： The detection rate of high-risk HPV-DNA in the normal group and CIN group （30. 77%, 66. 29% ） was significantly lower than that of cervical cancer group （96. 77% ） ; the detection rate of high-risk HPV-DNA in normal group （30. 77% ） was significantly lower than that of CIN group （66. 29% ） ; the detection rate of high-risk HPV-DNA in CIN I group and CIN Ⅱ Group （44. 83% , 55.93% ） was significantly lower than that of CIN III group （94.83%） ; the detection rate of high-risk HPV-DNA in CIN Ⅰ group （44. 83% ） was significantly lower than that of CIN II group （55.93%）, all with statistically significant differences （P 〈 0. 05）. Compared with the high-risk HPV-DNA load among all the groups, it was found that the high risk HPV- DNA load did not increase with the severity- of cervical lesions, and the diff

关 键 词：高危型 HPV-DNA 负荷量 宫颈病变程度 相关性 

分 类 号：R737.33[医药卫生—肿瘤]