Study of a Whitening and Anti-wrinkle Bio-functional Ingredient Based on Epigenetics  

作　　者：Zhao Xiaomin Zhao Yunshan Qu Xin 

机构地区：[1]Shanghai Technical Center, Ashland (China) Inc.

出　　处：《China Detergent & Cosmetics》2017年第3期48-53,共6页日用化学品科学（英文版）

摘　　要：Via quantitative polymerase chain reaction （qPCR） test method, the efficacy of hexapeptide-2 was studied, including silencing on miR-29 and increasing miR-218 expression which targeted collagen coding mRNA （message RNA） then decreases the collagen synthesis, and the latter silences the melanin synthesis related enzyme. Then the double blind spilt-face clinical test on 35 Asian people （18 male and 17 female） was conducted to evaluate the whitening and anti-wrinkle effect. The qPCR test showed that hexapeptide-2 can decrease miR-29 expression for human fibroblast of different age （P 〈 0.05）; and the result on human melanocyte showed that it can increase miR-218 expression （P 〈 0.05）. Immunostaining results showed that it can increase the expression of collagen I and III significantly （P 〈 0.05）. The double blind split - face test showed that 1% hexapeptide-2 displays significant efficacy for skin whitening, spots lightening, skin erythema and skin dullness improvement, as well as skin wrinkles reduction as comparing with that from the placebo side （P 〈 0.05）.Via quantitative polymerase chain reaction(qPCR) test method, the efficacy of hexapeptide-2 was studied, including silencing on miR-29 and increasing miR-218 expression which targeted collagen coding mRNA(message RNA) then decreases the collagen synthesis, and the latter silences the melanin synthesis related enzyme. Then the double blind spilt-face clinical test on 35 Asian people(18 male and 17 female) was conducted to evaluate the whitening and anti-wrinkle effect. The qPCR test showed that hexapeptide-2 can decrease miR-29 expression for human fibroblast of different age(P < 0.05); and the result on human melanocyte showed that it can increase miR-218 expression(P < 0.05). Immunostaining results showed that it can increase the expression of collagen Ⅰ and Ⅲ significantly(P < 0.05). The double blind split-face test showed that 1% hexapeptide-2 displays significant efficacy for skin whitening, spots lightening, skin erythema and skin dullness improvement, as well as skin wrinkles reduction as comparing with that from the placebo side(P < 0.05).

关 键 词：cosmetics additives ANTI-WRINKLE skin whitening EPIGENETICS microRNA 

分 类 号：TQ658[化学工程—精细化工]