利用气味分子TMT建立大鼠条件性位置偏恶模型  

Establishment of conditioned place aversion in rats by predator odor TMT

作　　者：陈琳[1,2] 王小红[3] 张婵溪 陈磊 翟海峰[1,2] CHEN Lin;WANG Xiaohong;ZHANG Chanxi;CHEN Lei;ZHAI Haifeng(Department of Pharmacology,School of Basic Medical Science,Peking University,Beijing,100191,China;National Institute on Drug Dependence,Peking University,Beijing,100191,China;School of Chinese Materia Medical,Beijing University of Chinese Medicine,Beijing,102488,China)

机构地区：[1]北京大学基础医学院药理学系,北京100191 [2]北京大学中国药物依赖性研究所,北京100191 [3]北京中医药大学中药学院,北京102488

出　　处：《中国药物依赖性杂志》2018年第4期263-266,287,共5页Chinese Journal of Drug Dependence

基　　金：国家自然科学基金(81271473)

摘　　要：目的:捕食者气味影响啮齿类动物的行为,本实验利用一种捕食者气味分子TMT并结合经典条件性位置偏爱(CPP)装置建立条件性位置偏恶模型。方法:将大鼠放置在CPP装置内,并在装置单侧给予TMT,探究急性暴露TMT对大鼠回避行为的影响;将大鼠放置在CPP装置单侧并暴露于TMT 20 min,重复暴露训练4 d后,再将大鼠放置在CPP装置内不给TMT,使大鼠在CPP测试箱内自由探索,通过测试大鼠在伴药侧和非伴药侧的停留时间,观测重复暴露TMT对大鼠条件性偏恶行为的影响。结果:大鼠在急性暴露于TMT后表现显著的回避行为,对给药侧表现位置偏恶;大鼠在重复暴露于TMT后,建立了TMT的负性效应与CPP装置给药侧相关联的记忆,表现出条件性回避行为,对伴药侧表现显著的位置偏恶。结论:通过将大鼠重复暴露于TMT并结合CPP装置能够建立稳定的条件性位置偏恶(厌恶记忆)模型;该模型可以在学习记忆相关研究中作为条件性位置偏爱(奖赏记忆)的对照模型。Objective： The predator odor molecule, TMT（ 2,5-Dihydro-2,4,5-trimethylthiazole）,has significant effects on the behaviors of rodents. In this study,TMT and a device for conditioned place preference（ CPP） are used to establish conditioned place aversion（ CPA） which can be used as a comparative model of CPP.Methods： Rats were put into the CPP device,and TMT was given in one side of the device to investigate the effect of acute exposure of TMT on the avoidance behavior. And then rats were exposed to TMT and put into one side of the CPP device for 20 minutes.After four days of repeated exposure training,rats were allowed to explore in CPP device freely. By testing the residence time in drug-paired box and none-paired box of rats,the effect of repeated exposure of TMT on the avoidance behavior of rats was assayed.Results： Rats showed significant avoidance after acute exposure to TMT. After repeatedly exposure to TMT,rats established a memory that TMT-induced negative effect was associated with one side of the CPP device,shown by a significant conditioned place aversion to the TMT-paired box.Conclusion： A stable CPA model can be established by repeated exposure to TMT with the CPP device. This conditioned aversion（ negative memory） can be used as a comparative model to CPP（ reward memory） in related researches.

关 键 词：捕食者气味 TMT 条件性位置偏恶 大鼠 

分 类 号：R749.6[医药卫生—神经病学与精神病学]